Prepare 25wt% aqueous solution of potassium fluoride ?

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What is the most appropriate approach to determine helix spacing to diameter ratio (S/D) in conical (tapered) helical piles?

Helix spacing to diameter ratio (S/D) is one of the determinant factors in the designation of helical piles failure mechanism and, consequently, the bearing capacity. In typical helical piles,...

29 September 2022 3,041 2 View

How to generate vertical boundary condition in MIKE 3?

Hello everyone, I would like to know how to generate vertical boundary condition for MIKE 3. I have HYCOM (volume data / .dfs3) and i am not sure how to extract vertical layer for boundary...

07 February 2021 8,728 3 View

Why higher OD in no substrate blank than inactivated enzyme blank?

Hi all, I am doing an Alkaline Phosphatase activity assay using PPi as substrate. I am using PiBlue Kit to measure amount of Pi generated at 620nm. I am getting high OD values in my no substrate...

08 September 2020 8,833 6 View

Visualizing cell pellet after FACS?

Hi all, I am sorting small number of cells (10,000-50,000)in 1.5 ml tubes using FACS. I am unable to see pellet of cells in the tube. I understand that it is difficult to observe pellet with such...

25 February 2018 2,154 6 View

How long can I keep rat's femur/tibia in 10% neutral buffered formalin for?

How long can I keep the bones in NBF before processing for histology and/or immunohistochemistry?

29 August 2016 9,695 2 View

What is the best method to remove soft tissues from a dissected rat's long bone?

I have tried scraping the soft tissues off using a scalpel but the whole procedure was a lengthy one. So I was wondering if anyone could tell me any other quicker way of removing the soft tissues...

22 August 2016 3,610 15 View

Which site of bone is preferable for studying the effect of chemical exposure on long bones?

I am trying to find out the effects of organic arsenic exposure on long bones in rats. However, it still baffles me as to which anatomical site of bone is more useful for research. I have come...

20 August 2016 875 3 View

What is the optimal method of measuring oxidative stress status in rat's femur?

Hi, I will be studying the effects of organic arsenic exposure on rat's long bone for my master's degree and I am planning to use the right femur to measure the lipid peroxidation status...

19 August 2016 4,535 3 View

Saffron micropropagation (need help) ?

I have worked micropropagation of saffron. I have a problem. Saffron bulbs explant growth a few day in first week on MS media then stop growth. After no growth no die. Stay stable. I tried all PGR...

04 August 2016 5,151 5 View

What can be Lineage-CD45+SCA-1+ positive Cells in brain?

Hi all, I am working on innate lymphoid cells and found that in our settings we have significant Lin-CD45+SCA-1+ cells. I further analyzed these cells for the expression of THY1, KLRG1, IL7ra and...

26 July 2016 982 4 View

Absorption coefficient of methane?

Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?

06 August 2024 980 5 View

How to calculate the molar proportions of the oxides from the XRF analyses?

02 August 2024 2,565 2 View

How to wash capsule filters (Membrane: polyethersulfone; pore size0.2u/0.45u) after filtration. can I wash by NaOH? if yes, what will be the molarity?

11 July 2024 8,969 1 View

Would it be possible to determine how many Cys I have based on Ellman's assay?

I want to predict the DAR ratio based on the number of cys available for conjugation to Cys residue. I use Ellman's assay to predict the concentration of Cys after TCEP reduction. I gain a...

02 July 2024 612 6 View

What is the best way to calculate the weight of each lipid component needed to achieve the desired molar ratios?

I'm currently working on preparing a 10 mL liposome solution containing HSPC, mPEG2000-DSPE, and cholesterol at a molar ratio of 55/40/5. can anybody advise on the best way to calculate the weight...

26 June 2024 1,135 0 View

Pls how can I combine CTAB and lactic acid for pretreatment of lignocellulosic material?

The percentage molar concentration of lactic acid and CTAB

20 June 2024 3,225 0 View

Why cells are visibly pink after DHE staining?

Good evening, I´m establishing a protocol for detecting ROS using DHE staining in Flow Cytometry using TK6 cells. They look visibly pink after staining them with 10 micro Molar DHE. Is that...

19 June 2024 7,339 0 View

Can somebody explain me about this "NaCl-KCl (8 g), a molar ratio of 1:1 and an eutectic temperature of 657 °C"?

I want to prepare a photocatalytic material Bi3TiNbO9 nanosheets and the authors mentioned NaCl-KCl (8 g), a molar ratio of 1:1 and an eutectic temperature of 657 °C as a one component. So, kindly...

04 May 2024 9,282 1 View

How to fix the molarity of 2 analytes to be interacted in Gauss View using DFT ?

I am trying to optimise the molar ratio of 2 analyte (For example A and B) during a reaction. For this purpose I want to optimise there molar ratio. For example A:B = 1:2, 1:3, 1:4, etc. Which...

28 April 2024 5,775 2 View

How can I get my desired DNA copies?

Hi all, I want to have 301 copies/ul for my downstream experiments. The sample I am using is a purified plasmid, of which I know the concentration (ng/ul), volume (ul), and molarity(g/mol). How...

20 April 2024 2,468 4 View