Why higher OD in no substrate blank than inactivated enzyme blank?

Hi all, I am doing an Alkaline Phosphatase activity assay using PPi as substrate. I am using PiBlue Kit to measure amount of Pi generated at 620nm. I am getting high OD values in my no substrate...

08 September 2020 8,708 6 View

Visualizing cell pellet after FACS?

Hi all, I am sorting small number of cells (10,000-50,000)in 1.5 ml tubes using FACS. I am unable to see pellet of cells in the tube. I understand that it is difficult to observe pellet with such...

25 February 2018 2,084 6 View

How long can I keep rat's femur/tibia in 10% neutral buffered formalin for?

How long can I keep the bones in NBF before processing for histology and/or immunohistochemistry?

29 August 2016 9,628 2 View

What is the best method to remove soft tissues from a dissected rat's long bone?

I have tried scraping the soft tissues off using a scalpel but the whole procedure was a lengthy one. So I was wondering if anyone could tell me any other quicker way of removing the soft tissues...

22 August 2016 3,507 15 View

Saffron micropropagation (need help) ?

I have worked micropropagation of saffron. I have a problem. Saffron bulbs explant growth a few day in first week on MS media then stop growth. After no growth no die. Stay stable. I tried all PGR...

04 August 2016 4,969 5 View

What can be Lineage-CD45+SCA-1+ positive Cells in brain?

Hi all, I am working on innate lymphoid cells and found that in our settings we have significant Lin-CD45+SCA-1+ cells. I further analyzed these cells for the expression of THY1, KLRG1, IL7ra and...

26 July 2016 908 4 View

Mice deficient in ILC3?

Dear all, Are there any mice strain available which are deficient specifically in group 3 or group 2 ILCs? best, Arshed

26 April 2016 5,313 3 View

How to get/count refractive index in gasses?

20 February 2021 8,202 6 View

Want to ask about equivalence ratio?

09 February 2021 5,273 2 View

In Michaelis-Menten Kinetics, why K(subscript)On have units (mol^-1)(time^-1)?

where K(subscript)On is the rate of Enzyme (E) + Substrate (S) going to Enzyme-Substrate complex (ES). I think I understand why K(subscript)Off and K(subscript)catalysis have units of time^-1....

11 January 2021 649 8 View

Do chemical and physical properties influence on the size of a polymer particle?

I would like to know if the properties of a polymer impact on the size of the particle it forms. I'm working with different polyssacharides (alginic acid from different seaweed), which are...

29 December 2020 7,333 6 View

How to make solution with molar ratio 1:40?

Currently i'm working to develop encapsulation using ferritin, based on many journals they always wrote the molar ratio between drugs and ferritin, for example 1:40, how to know the molarity that...

22 December 2020 6,582 2 View

Having issues understanding the particles/mL to molarity conversion, I've seen many different way but non of them seem to match one an the other?

I have 68nm spherical Au NPs, which gave me a concentration of 1.22 x 10^9 particles/mL. How can I calculate the molarity without the need for TEM?

22 November 2020 8,850 3 View

What solvent can I use to prepare a higher concentration of 5-Bromouracil?

I am standardizing a UHPLC method for separation of thioguanine phosphate metabolites. For this, I want to use 5-Bromouracil (5-BU) as an internal standard, however I am unable to prepare solution...

15 November 2020 4,982 3 View

How storage capacity for clathrate hydrate is calculated?

Storage capacity of hydrate solely depends on molar volume of empty hydrate lattice because rest of the terms get canceled (even moles of gas consumed). However, if we take temperature constant,...

16 October 2020 720 2 View

Please suggest that the way of obtaining the concentration (in mol) from the gas mixture (N2=98%, Ethylene=1% and Butadiene=1%) is correct or not?

I have a gas mixture (N2=98%, Ethylene=1% and Butadiene=1%). I want to obtained the molar concentration of each components for 1mL volume of the gas mixture. For that purpose, I have used the...

29 September 2020 6,233 1 View

How can I make a HgSO4 1M solution?

Hi everyone I want to make a HgSO4 1 Molar solution for deposition (electroplating) in my electrode, but HgSO4 is not soluble in water! What can I do !? Because My other solutions are all in water.

14 September 2020 3,461 3 View