I have worked micropropagation of saffron. I have a problem. Saffron bulbs explant growth a few day in first week on MS media then stop growth. After no growth no die. Stay stable. I tried all PGR combination with MS but results same. Can you give me opinion ????
try to alter environmental condition, decrease temperature and humidity of culture.
Please have a look at this links
https://www.researchgate.net/publication/275965535_In_Vitro_Micropropagation_of_Saffron
https://www.researchgate.net/publication/289609027_Advances_in_studies_on_tissue_culture_of_saffron_crocus_sativus_L
http://www.ncbi.nlm.nih.gov/pubmed/22892688
Good luck
Article In Vitro Micropropagation of Saffron
Article Advances in studies on tissue culture of saffron (crocus sativus L.)
which type of sterilization method are using??
and culture conditions???
you can check our paper,
l hope you can find something useful information.,
Thanks for your help. I used MS(Murashige and Skoog-Duchefa) regeneration medium supplemented with 3 % sucrose, 0.7% agar (Duchefa), autoclaved at 121 oC under pressure of 1.2 kg/cm 2 for 20 minutes. The pH of the medium was adjusted to 5.6-5.8 with 0.1 N KOH or HCl before autoclaving. All PGR (BAP, NAA, 2,4D, IBA, IAA) autoclaved. Temperature 24 C. 16 h light, 8 h dark. I will send photos about growth.
- Badges
- Science topic
- Similar topics
- Biological Science
- Botany