Would using an asRNA for repressing transcription of a high expression Listeria monocytogenes non-coding RNA harbored in a plasmid be a viable option?

Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...

09 February 2021 4,994 1 View

SDS-Gel stacking gel polymerization issues?

Can anyone provide any insight as to why my Tris/Gly SDS-Gels are polymerizing slowly? This especially applies to my stacking gel (image attached) both 4% and 6%. The stacking gel wells polymerize...

24 January 2021 3,791 4 View

Will rat colon cancer cells (DHD/K12) derived from BDIX rats grow in Sprague Dawley ?

we plan to induce colon cancer by injection. Cell lines derive from BDIX rats, but our experimental setting would favour SD rats. any experience?

23 January 2021 1,437 2 View

I am interested in sequencing small RNAs in bacteria but enriching for small, weakly expressed transcripts seems to be a challenge. Any ideas?

Assays for bacterial small RNA sequencing

06 January 2021 4,549 2 View

What is the white layer on my fermented natto bean paste?

Dear all, Good day! Recently i tried to ferment my blended soya bean powder mixed with Natto bacteria culture in a Natto maker (containing some water) at around 45 degree celsius overnight....

23 November 2020 7,105 20 View

How do I extract mice brains for multiple experiments?

I am looking to complete western blot experiments and IHC experiments on the same cohort of mice. Is it possible to Harvest the mouse and use the right hemisphere for IHC and the left for western...

08 September 2020 5,436 3 View

Why the need to have CIE formula when color difference can be measured with RGB values?

Dear all, Recently i have been trying to measure colour difference between my samples. I am a little confused as to why i cannot just use the basic colours - i.e., red, green and blue to measure...

02 August 2020 7,375 2 View

Does anyone know of an Antibody against against Mouse Microglia trf2 that is good for Immunofluorescence and Westernblot?

Hi, I am studying the knockdown of Trf2 protein in Murine microglia cells invitro and wish to demonstrate this knockdown via immunofluorescence and Western blot. A dependable Trf2 antibody and...

02 June 2020 8,682 3 View

Are their any articles on the methodology of theory development?

Can anyone suggest articles on the methods of theory development? There are tons of articles on the methods of experimental design and statistical analysis. However, I have a hard time finding...

31 May 2020 3,264 2 View

Should I use log transformed data for correction then revert it back?

Hi, I'm trying to merge two chlorophyll datasets from different sensors by applying linear regression on their data overlap. The journal that I'm following log transformed (base 10) the data from...

02 April 2020 871 7 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View