Whats the best culture medium/agar to grow fungi only?

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Error in solve.default(V, tol = .Machine$double.eps) : Lapack routine dgesv: system is exactly singular: U[28,28] = 0 how to resolve?

hello everyone, Anyone can help me fix that error that occurred when I was running PGLS model in caper package .

21 February 2024 3,475 0 View

Is there a preferred method of reporting GPS coordinates in field biology?

Does it matter how GPS coordinates are reported in field biology - as in, is there a general preference between reporting GPS data using sexagesimal degrees (Degrees, Minutes, Seconds) or decimal...

12 January 2024 9,045 2 View

What methods can be done to ensure that laboratory workers follow proper quality control procedures?

One of the factors that can affect the quality control of the laboratory is the laboratory workers. However, some laboratory workers do not follow the procedures for various reasons. What methods...

03 September 2023 3,548 3 View

What are good approaches to research etymologies?

I am considering doing some research on the etymological origins of certain words related to student government and student leadership, particularly words such as "student", "pupil", etc., to...

08 August 2023 4,539 6 View

How do you fix a stuck pump on a Biotage Isolera?

I am currently trying to just prime my Biotage Isolera but it keeps giving me an error saying pump A is stuck. I tried cleaning the check valve to see if that will clean anything, it did not....

22 June 2023 569 2 View

Are academic book reviews worth it?

Do academic hiring committees consider book reviews? I enjoy doing book reviews because I get free books, small publications, and it allows me to stay up to date on recent scholarship in my...

21 May 2023 7,997 9 View

Does anyone know how, after importing a correlation matrix in R, I can specify the # of observations?

22 February 2023 9,972 1 View

How difficult was it for you to obtain a tenure-track position?

What was your experience searching for academic jobs for the first time? Were you able to obtain a tenure-track position? How many jobs did you apply to, where did you apply, and what was the...

14 January 2023 4,928 5 View

Why do we have to do a seismic model before carrying out inversion process?

A geophysical model is obtained before inversion process.

11 December 2022 4,645 3 View

What is the significance of carrying out wavelet extraction during seismic data processing petrophysical analysis?

The above question is part of the workflow for inversion process using 3D seismic wave data

11 December 2022 8,394 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

Belle Damodara Shenoy

MEA

Anil Kumar

Potato dextrose agar (PDA) with some concentration of antibiotics to prevent the bacterial growth

Sansrity Sinha

CDA(czapex Dox Agar) and PDA (Potato dextrose agar) are most preferred media for filamentous fungi with antibiotics specific to fungi to be grown. U can also try oatmeal agar.

Akermi moulay m'hammed

CDA(czapex Dox Agar) and PDA (Potato dextrose agar) are most preferred media for filamentous fungi with lactic acid 4%.