I am prepareing the universal Sample diluent for ELISA use. after refering the patents and papers i found every whare they used KCL and casein hyrolase so i want to know the reasion. kindly help
We are developing the T3 streptavidin based assay. Currently we tried different T3 mab. These mab gives good results when coated directly on ELISA plate. But after biotinylation, mabs behaves...
04 May 2015 2,902 8 View
02 March 2014 8,015 2 View
We are in the phase of developing the total T3 streptavidin based ELISA kit, in which we are facing problems: Standards are comming in range, samples are also in the range but when we use the...
08 September 2013 7,813 2 View
We are using Sigma kit for this conjugation. But we dont know how many Biotin molecules attached to one IgG (Mouse monoclonal)
08 September 2011 5,643 0 View
This article is mine. It was republished by unknown person using an address (Wilson N Jones Regional Center, Sherman Texas USA. I have a patent.
10 August 2024 7,528 5 View
I am developing an ELISA kit for rotavirus A (G1P8 and G1P6). Does anyone know the weight (ng) of these two strains? Or to help me find their weight?
29 July 2024 3,138 2 View
hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...
16 July 2024 9,044 10 View
I placed mice in metabolic cages to collect urine samples for analysis. However, the majority of the samples have dried up. Is there any way to retrieve the urine in those tubes without...
14 July 2024 6,724 0 View
Dear academician friends, I have a question about econometrics. I evaluated the relationship between the number of researchers in the health field and patents with a balanced panel analysis over...
03 July 2024 3,465 5 View
"Dear Researchers, I am currently working on a Nanotechnology project and am exploring the process of drafting a patent to protect our innovations. Could anyone here share insights or tips on how...
02 July 2024 9,627 2 View
Anyone have a protocol for making cell lysate from rat cardiomyocyte pellets to run an ELISA using PBS as the buffer?
23 June 2024 2,264 4 View
If cells obtained from different passages, with different plating but harvested on the same day are used in a single plate to take an ELISA measurement (with the given standards and treated...
17 June 2024 6,705 1 View
I used two standards (one came with the ELISA kit and the other I made myself, by preparing a series of known concentrations of the target analyte) on the same plate in my ELISA run. Why are the...
15 June 2024 5,109 1 View
Dear fellow researchers, We are conducting an ELISA with the "Cattle IGF 1 (Insulin-like growth factor) ELISA Kit" (Cat: ELK5630). Samples and reagents were prepared following the instruction...
13 June 2024 9,473 0 View