Sandeep Kulkarni

5 Questions 9 Answers 0 Followers

Questions related from Sandeep Kulkarni

Whate is role of KCL in ELISA sample diluent?

I am prepareing the universal Sample diluent for ELISA use. after refering the patents and papers i found every whare they used KCL and casein hyrolase so i want to know the reasion. kindly help

11 November 2017 8,918 0 View

Biotin conjugation problem with MAb: can any one help?

We are developing the T3 streptavidin based assay. Currently we tried different T3 mab. These mab gives good results when coated directly on ELISA plate. But after biotinylation, mabs behaves...

05 May 2015 2,871 8 View

I want to buy T3 antibodies for T3 ELISA standardization - any thoughts?

We are developing the T3 streptavidin based assay. Currently we tried different T3 mab. These mab gives good results when coated directly on ELISA plate. But after biotinylation, mabs behaves...

03 March 2014 7,996 2 View

Streptavidin based TT3 ELISA?

We are in the phase of developing the total T3 streptavidin based ELISA kit, in which we are facing problems: Standards are comming in range, samples are also in the range but when we use the...

09 September 2013 7,797 2 View

Dear all please tell me how I can control Biotin-Protein ratio while doing Biotin conjugation? Is there any specific method/reagent available?

We are using Sigma kit for this conjugation. But we dont know how many Biotin molecules attached to one IgG (Mouse monoclonal)

09 September 2011 5,619 0 View