17 Questions 36 Answers 0 Followers
Questions related from Fawaz Al-Shaheri
Dear all, I am doing a targeted methylation analysis on circulating tumor DNA. After bisulfite conversion, I do Whole Bisulfitome Amplification, since DNA concentration after the bisulfite...
26 September 2022 8,062 0 View
Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?
24 February 2021 4,129 3 View
Hi everyone, We are doing a coculture of cancer cell lines and monocytes (THP-1). As a control, we need to cultivate THP-1 in the context of normal cells. For this experiment, we need to polarize...
17 February 2021 9,701 5 View
Hi everyone, During small RNA library preparation, I run the samples in 4% agerose gel to select the band corresponding to my target. I established beforehand the protocol and it works fine....
03 February 2021 5,170 2 View
Hi everyone, I am doing a quality control for NGS libraries using bioanalzyer 2100 from Agilent. The machine worked for me for almost two years without any problem, with regular cleaning and...
30 November 2020 1,093 0 View
Hello everyone, I am preparing a manuscript for a journal which indicates in the authors guidelines that the citation and referencing style should look like the following: Citation in the text...
23 September 2020 7,047 6 View
Hello everybody, I am currently extracting total RNA including microRNA from serum using miRNeasy Serum/ Plasma kit Catalog Number 217184 from Qiagen. Within the procedure, we add a Spike -In...
14 August 2019 2,308 2 View
I working with pancreatic cancer cell lines PT45P1 and would like to know whether there is a better test rather than wound healing assay to assess the cellular proliferation after and before...
07 August 2019 6,309 9 View
I am planning to extract the whole serum protein from serum samples for further microarray analysis. what is your recommendations please. a good protocol is highly appreciated. Thanks in advance
17 September 2018 4,020 3 View
I am studying the methylation status of APC gene in colorectal patients. My practical approach starts with bisulfite conversion of DNA and then amplification using methylation specific PCR....
11 September 2017 2,748 2 View
a project in our group investigating the association between specific and drug resistance. One of these SNPs has been reported that the wild type alleles GG have been altered into TT. Using...
01 June 2017 3,108 3 View
We need pre-malignant colorectal adenoma cell lines to study the effect of mutation in malignant transformation(tubular adenoma, villous adenoma and tubuvillous adenoma cell lines). I have been...
01 January 1970 1,107 3 View
We have identified a specific sequence for a gene we are working on. Using PCR, a band less than expect was identified. So, we planned to excise and purify the band using gel purification kit....
01 January 1970 4,223 3 View
We are extracting DNA from aged blood samples using Qiagen DNA extraction Kit. Unfortunately, the DNA concentration that we've got is 5-6 microgram/ microliters at 260/280. Do you have any...
01 January 1970 8,954 5 View
I am planning to do functional analyses for a novel mutation to investigate its effect using cell based cloning. The data from literature indicate that pET+25b is the best vector to create the...
01 January 1970 1,612 3 View
I am working in the methylation status of some genes in cancer patients. We recruited clinical samples from patients with and without cancer. We designed methylation specific primer and...
01 January 1970 4,055 2 View
We are planning to detect the number of GT repeats in the promotor of a specific gene. Our specific aim is to correlate the number of repeats with protein levels measured previously wit ELISA. We...
01 January 1970 715 3 View
