Fawaz Al-Shaheri

15 Questions 31 Answers 0 Followers

Questions related from Fawaz Al-Shaheri

Targeted methylation analysis of cfDNA?

Dear all, I am doing a targeted methylation analysis on circulating tumor DNA. After bisulfite conversion, I do Whole Bisulfitome Amplification, since DNA concentration after the bisulfite...

26 September 2022 8,052 0 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

24 February 2021 4,087 3 View

How much of TNF-Alpha is added to transform THP-1 into M1 macrophage?

Hi everyone, We are doing a coculture of cancer cell lines and monocytes (THP-1). As a control, we need to cultivate THP-1 in the context of normal cells. For this experiment, we need to polarize...

17 February 2021 9,690 5 View

What could be the possible causes of DNA degradation after extraction from 4% gel?

Hi everyone, During small RNA library preparation, I run the samples in 4% agerose gel to select the band corresponding to my target. I established beforehand the protocol and it works fine....

03 February 2021 5,140 2 View

What is this citation style called?

Hello everyone, I am preparing a manuscript for a journal which indicates in the authors guidelines that the citation and referencing style should look like the following: Citation in the text...

23 September 2020 7,024 6 View

What would be the effect of Spike-In control in microRNA Next Generation Sequencing results?

Hello everybody, I am currently extracting total RNA including microRNA from serum using miRNeasy Serum/ Plasma kit Catalog Number 217184 from Qiagen. Within the procedure, we add a Spike -In...

14 August 2019 2,299 2 View

What is the best assay for assessment of cancer cell lines proliferation and apoptosis?

I working with pancreatic cancer cell lines PT45P1 and would like to know whether there is a better test rather than wound healing assay to assess the cellular proliferation after and before...

07 August 2019 6,292 9 View

What the best protocol for protein extraction from human serum?

I am planning to extract the whole serum protein from serum samples for further microarray analysis. what is your recommendations please. a good protocol is highly appreciated. Thanks in advance

17 September 2018 3,999 3 View

How can we check the specificity of methylation Specific Primers?

I am studying the methylation status of APC gene in colorectal patients. My practical approach starts with bisulfite conversion of DNA and then amplification using methylation specific PCR....

11 September 2017 2,728 2 View

Where we can find Colorectal Adenoma cell lines?

We need pre-malignant colorectal adenoma cell lines to study the effect of mutation in malignant transformation(tubular adenoma, villous adenoma and tubuvillous adenoma cell lines). I have been...

01 January 1970 1,091 3 View

How can we predict that a particular sequence is a pseudogene?

We have identified a specific sequence for a gene we are working on. Using PCR, a band less than expect was identified. So, we planned to excise and purify the band using gel purification kit....

01 January 1970 4,209 3 View

How we can improve the DNA concentration extracted from aged samples?

We are extracting DNA from aged blood samples using Qiagen DNA extraction Kit. Unfortunately, the DNA concentration that we've got is 5-6 microgram/ microliters at 260/280. Do you have any...

01 January 1970 8,936 5 View

What is the most suitable restriction enzyme we should use to insert the MUTYH into plasmid vector pET+25b?

I am planning to do functional analyses for a novel mutation to investigate its effect using cell based cloning. The data from literature indicate that pET+25b is the best vector to create the...

01 January 1970 1,600 3 View

What is the cause of nonspecific bands in methylation specific PCR?

I am working in the methylation status of some genes in cancer patients. We recruited clinical samples from patients with and without cancer. We designed methylation specific primer and...

01 January 1970 4,039 2 View

What is the best method for detecting the number of GT repeats in the promotor of a gene?

We are planning to detect the number of GT repeats in the promotor of a specific gene. Our specific aim is to correlate the number of repeats with protein levels measured previously wit ELISA. We...

01 January 1970 704 3 View