We are using some enzymes to test in the laboratory. Trying to figure out the best method treat the waste water. The actual chemical we using are testing with enzyme based drain cleaners. Need guidance and suggestions..
Thank you.
I can think of a few ways, but I don't know if they will be practical for your application.
1. Ultrafiltration using membranes that have pore sizes too small for the proteins to pass through. Expensive and slow.
2. Putting the water over an ion exchange resin. This requires that the ionic strength (saltiness) of the water be as low as possible, and making sure the pH is in the right range. With large ion exchange membrane cartridges, this could be a relatively fast procedure, and the membrane cartridges can be regenerated for reuse.
3. Acid or base denaturation, which will cause the proteins to aggregate so that they can be removed by filtration or centrifugation. The acid or base would have to be neutralized again for disposal.
4. Proteolytic digestion. This involves adding more enzymes to degrade the enzymes as well as themselves, leaving only peptide fragments. Takes time and may be expensive.
Dear Aravindha Loganathan ,
Without knowing the quantity and matrix (composition and parameters) of this lab wastewater aside from the enzymes, it would be hard to determine the most suitable method to remove or destroy these enzymes. Hence I could only advise in more general terms. I agree in principle with the 4 methods listed by Adam B Shapiro . However applying separation methods based on membrane (nano) filtration or ion exchange on raw wastewater would probably not be the best choice resulting in membrane/resin fouling and an active enzyme concentrate which still needs to be treated further (denatured or broken down). Hence I would propose a 5th method which is advanced oxidation using ozone O3 + hydrogen peroxide H2O2 (aka peroxone) to break down and partially oxidize these enzymes along with other organic compounds in the lab wastewater.
The solution no 6 could be the simplest but depends on stability of the enzymes you are using and amount of wastes. Temperature increase or even boiling your waste...
Adsorption could be a beneficial one regarding this particular case, you can follow the links
1. Article Waste biomass derived chitosan-natural clay based bionanocom...
2. Article Adsorptive abatement of Pb2+ and crystal violet using chitos...
3. Article Fabrication of Nano Composite Membrane Filter from Graphene ...
4. Article Simultaneous abatement of Ni2+ and Cu2+ effectually from ind...
5. Article Removal of dye and heavy metals from industrial wastewater b...
6. Article Extraction of Nano-cellulose from Banana Rachis (Agro-waste)...
For removal of traces of bio enzymes in waste water,
Create float beds with plants growing vjz cayhna indicated sp.
Irrigate water in these beds, on recirculation mode, enough sun light Etc
With 2- 3 days HRT, may get normal water.. wll wshs..
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