How to isolate Marine Vibrio Phage DNA?

27 May 2021 0 5K Report

Dear Researchers,

I am cultivating Vibrio Phages in TSB media (20PPT salt). First, when i treat with DNase and RNase it is unable to remove complete 100% DNA (Host DNA). Any suggestion, how to remove complete DNA. Further, when i treat with Proteinase K (50ug/ml) and proceed with P:C:I extraction. The concentration is 200-500ng (5 ml sample). In gel , no bands. Could you suggest me how to improve the yield...whether salt is affecting Proteinase K activity.

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