I have depolymerized byproducts and commercialized products. I doubt how to calculate yield percentage using HPLC data. Can anyone give me an answer? It will be helpful in my research career. Thank you
Loganathan .M Divide the product concentration obtained from the HPLC data with the sole substrate concentration from which the product is formed and convert the obtained yield to percentage.
Noticeable , When using HPLC data, ensure that the peaks you're integrating correspond to the compound of interest and that your calibration curve is accurate for quantification. By comparing the area under the curve (AUC) of the compound in the sample to the AUC of known standards, you can determine the concentration of the compound and use it to calculate the yield.
First, you need to develop an HPLC method with which your product can be well separated from the by-products. With this HPLC method, you generate a standard curve using the standard materials (the pure product). In this standard curve, the amount values of the standard are used for the X-axis and the corresponding peak area values for the y-axis. Then you run HPLC analysis for your samples. You will get the peak area for each of your samples (sample retention time is the same as the standard). Then, using the standard cure, you can calculate the amount of the studied material in your samples
To calculate yield percentage from HPLC data: identify peaks for byproducts and products, measure peak areas or heights, and use the formula: Yield Percentage = (Amount of Product / Amount of Starting Material) * 100%. Ensure accurate identification and integration of peaks. Replicate experiments for reliability. Adjust calculations for sample purity if necessary. This analysis provides insights into reaction efficiency and product formation, aiding in research conclusions and process optimization in fields like chemistry, pharmaceuticals, and materials science.
- Badges
- Science topic
- Similar topics
- Engineering
- Materials Engineering
- Powders