You are carrying out virus infections. Your stock contains
5 x1010 pfu/ml. What volume of this stock would you use
to infect a tissue culture flask containing 2 x107 cells at
an multiplicity of infection of 5 pfu/cell?
Malcolm Nobre
Hello Shuvo Saha
You will have to use two formulae.
Formula No1:
Number of cells x desired MOI = total PFU (Plaque Forming Units) needed.
So, in your case
2 x10^7 cells x 5 pfu/cell = 10 x 10^7 (Total PFU desired)
Then, use Formula No 2:
(Total PFU desired) / (PFU/ml) = total ml of virus needed to reach your desired dose.
So, in your case
10 x 10^7 (Total PFU desired) / 5 x10^10 pfu/ml = 2 x 10^-3 = 0.002ml or 2ul.
Add 2.0ul of the stock to the tissue culture flask containing 2 x10^7 cells and label it as 5 MOI.
Best.
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