What type of contamination?

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Can we use technical replicates for statistical analysis of multiplex cytokine data?

I have done an experiment using luminex technique, measuring 46 analytes. but my sample size is less (N=6, for case and control each). However, i have put up the experimental set in duplicates....

31 July 2024 3,664 3 View

How to analysis data from questionairre ?

How to qualitative data analysis from open questionnaires to a positive outcome

03 June 2024 9,829 3 View

coaching effectiveness surevy questionairres ?

Can anyone please share such questionaires for a project study please

29 April 2024 5,946 5 View

What are the coaching effectiveness surevy questionairres ?

Effectiveness of coaching is possible . Can anyone enlighten please on this subject matter ,Required for a project.

29 April 2024 8,536 2 View

What are the key aspects, benefits, and downsides of System Dynamics if applied to business model innovation for digital transformation?

12 April 2024 2,002 4 View

How will Blockchain, Generative AI, VR, AR, & other disrupt technologies impact Smart Meters in future on utilities, consumers, & regulators?

I am a research scholar at SSBM Geneva, Switzerland, trying to develop a System Dynamics Model for studying the long term impact of smart metering and smart grids on electric utilities,...

26 March 2024 2,524 3 View

How to design a controlled discharge circuit for a capacitor?

I want a circuit that will discharge a capacitor after some voltage say 100V. The capacitor will charge periodically upto 150 V. Whenever the voltage exceeds 100V I need to discharge it and the...

26 February 2023 6,689 3 View

How to analyze a oligosaccharide mixture by HPLC (UV detector)?

We need to identify a mixture of chitooligosaccharides (COS). Our previous TLC analysis showed our COS mixture consists of N-acetyl D-glucosamine (GlcNAc), Diacetyl chitobiose (GlcNAc)2,...

25 October 2021 2,145 6 View

Analysis of a carbonate (calcite) vein sample yielded d18O of 6.09 (V-SMOW) and d13C of -4.23 (V-PDB), and only 47 ppm Sr. Can it be carbonatite?

Analysis of a carbonate (calcite vein) sample yielded d18O of 6.09 (V-SMOW) and d13C of -4.23 (V-PDB), and only 47 ppm Sr. Stable isotopic data indicate 'mantle signature', while Sr abundance is...

05 October 2021 2,059 6 View

Antibody specific to placental exosomes??

I am struggling with isolation of placental specific exosomes. I have tried invitrogen polyclonal anti-palp antibody in comparison between male PLAP+, Non-pregnant PLAP+ (NP) and pregnant PLAP +....

01 July 2021 9,019 0 View

Is skin yellowness an numerical or ordinal variable?

I have a response variable called skin yellowness, which I will measure via a scored color chart, whereby 1 is pale yellow and 15 is orange. I'm not sure if this counts as an ordinal variable,...

11 August 2024 4,792 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,822 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,104 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 639 2 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 806 3 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View