What is the best suited direct protocol for miRNA isolation from exsosome?

I want to isolate exosomal miRNA only, for that do I need to first isolate total RNA and then go for miRNA isolation? or can I directly isolate miRNA from exosome and do the analysis? if yes...

08 December 2020 5,803 4 View

For electrochemical capacitors which type of specific capacitance is more technically preferable to represent?

Capacitance of electrochemical capacitors generally represented in relative scale, i.e. the units are represented either related to electrode area or the mass of active material. In most of the...

22 June 2019 8,243 5 View

What are the usefulness of Support Vector Machine Learning?

What are the usefulness of Support Vector Machine Learning?

03 May 2018 1,312 3 View

Why Id/Ig ratio increases after reduction of Graphene oxide and Ad/Ag ratio reduces for the same ?

Most of the time I have observed that Id/Ig ratio increases after reduction of GO and and Ad/Ag ratio decreases [where A denotes area under the peak]. Could you please help me by sharing your...

30 January 2018 7,220 5 View

Can anyone suggest me an online tool for drawing the miRNA networks with their target genes and pathways?

I have a set of novel miRNAs with their predicted target genes and pathways. Now i have to represent that data in pictorial form. Please suggest me which online drawing tool can be used for doing...

20 December 2017 7,169 2 View

Is it possible to use agilent 4156c semiconductor parameter analyzer as potentiostat for three electrode electrochemical measurement ?

I want to use agilent 4156c SPA as potentiostat for three electrode electrochemical measurement. It is possible to specify different scan rates (V/Sec) and potential windows (V) by this...

07 November 2017 7,421 2 View

Is it possible to find a Eulerian path or Eulerian cycle in polynomial time in a directed multigraph with self loops?

20 September 2014 2,498 2 View

No title

Using Gaussian 09

18 May 2014 7,786 2 View

rectal length

what is the correct length of the human rectum?

17 March 2010 5,178 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

No title

02 March 2021 3,060 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View