What is the best suited direct protocol for miRNA isolation from exsosome?

I want to isolate exosomal miRNA only, for that do I need to first isolate total RNA and then go for miRNA isolation? or can I directly isolate miRNA from exosome and do the analysis? if yes...

08 December 2020 5,803 4 View

For electrochemical capacitors which type of specific capacitance is more technically preferable to represent?

Capacitance of electrochemical capacitors generally represented in relative scale, i.e. the units are represented either related to electrode area or the mass of active material. In most of the...

22 June 2019 8,243 5 View

What are the usefulness of Support Vector Machine Learning?

What are the usefulness of Support Vector Machine Learning?

03 May 2018 1,312 3 View

Why Id/Ig ratio increases after reduction of Graphene oxide and Ad/Ag ratio reduces for the same ?

Most of the time I have observed that Id/Ig ratio increases after reduction of GO and and Ad/Ag ratio decreases [where A denotes area under the peak]. Could you please help me by sharing your...

30 January 2018 7,220 5 View

Can anyone suggest me an online tool for drawing the miRNA networks with their target genes and pathways?

I have a set of novel miRNAs with their predicted target genes and pathways. Now i have to represent that data in pictorial form. Please suggest me which online drawing tool can be used for doing...

20 December 2017 7,169 2 View

Is it possible to use agilent 4156c semiconductor parameter analyzer as potentiostat for three electrode electrochemical measurement ?

I want to use agilent 4156c SPA as potentiostat for three electrode electrochemical measurement. It is possible to specify different scan rates (V/Sec) and potential windows (V) by this...

07 November 2017 7,421 2 View

Is it possible to find a Eulerian path or Eulerian cycle in polynomial time in a directed multigraph with self loops?

20 September 2014 2,498 2 View

No title

Using Gaussian 09

18 May 2014 7,786 2 View

rectal length

what is the correct length of the human rectum?

17 March 2010 5,178 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View