Hi,

I have attached a protocol.

Please let me know if you have better results

Thanks

Angel

Hi

Thank u Angel

I tried it but no plaques can be seen

As the E1 gene is deleted from the virus

So do you have any alternative way ?

Thanks

Do you have any reporter gene expressed by the vector, like GFP or beta-gal? Without fluorescent or other labelling plaques can be difficult to relably recognize, which can lead to inconsistent results in repeated experiments.

Dilution factor also matters, if you count the wells with higher dilutions, where you have only a few plaques, the error will be high. Go for lower dilutions, i.e. for higher number of expected plaques so your error will be lower.