13 Questions 10 Answers 0 Followers
Questions related from Ashmenta Robrams
Hi I'm trying to generate murine DCs from NSG HLA A2 mice ,,, using 20ng GM-CSF However, all what I get is MHCII neg, F4/80 hi, which show they are macrophages ,,, So I wonder can I generate...
27 March 2019 1,201 3 View
Hi every one I wonder if I can differentiate between M1 and M2 macrophages in mouse tumours by using the following markers only? CD11b, CD11c, Ly6C, Ly6G, F4/80, MHCII If not ,,,which Ab I...
21 June 2018 7,333 2 View
what is the percentage of Her2 overexpression in PDAC? Accoring to Yamanaka et al (1993) HER2/neu immunoreactivity was expressed in 34 of 76 (45%) pancreatic carcinomas. Northern blot...
10 June 2018 7,079 1 View
Hi I need to infect dendritic cells derived from mouse bone marrow with adenovirus then co culture them with T cells I'm wondering how much time the dendritic cells need to process the virus...
06 May 2018 9,665 2 View
Hi I need a good panel for my FACS experiment which I 'll differentiate (mature DCs- which pulsed with peptide) and immature DCs ? Thanks
05 May 2018 2,166 3 View
Hi everyone I'm trying to titre some stocks of adenovirus that I isolated from infected 293a and I need a protocol to see the infection efficiency as the plaque forming assay However, I tried...
25 February 2018 7,090 3 View
Hi I give my mice 15mg/kg of a specific drug So how many nanomolar I give to my mice If the drug's formula weight is 439.52g/Mol ?? Thanks
28 January 2018 5,833 3 View
Hi I'm trying to generate human dendritic cells from cryopreserved leukopheresis,, but it does not work ,, I just get few cells ,,, I just thaw them and leave for 2 hr in incubator then wash x3...
28 November 2017 769 8 View
Hello everyone I wonder how can I pulse my mouse splenocytes with virus to be presented on MHC for co-culture experiments how many particles is enough for pulsing ,,, 10 virus / 1 cell? 50virus...
26 July 2017 4,243 2 View
Hi everyone I need a protocol to titer my adenovirus please? Something other than Adeno-X™Rapid Titer Kit please thanx
26 July 2017 2,969 2 View
Hi What does the different shape of cells mean, invivo subcuatneous, cells are from single sorting spindle shape and round shape of pancreatic cancer cell line from KPC mouse ? thanks
21 May 2017 3,024 1 View
Is there any difference when I find Monocytic or Granulocytic myeloid-derived suppressor cells ( MDSCs ) in the tumours? Thanks
18 April 2017 2,198 1 View
I need to test the proliferation ability of anti-Her2 CAR T cells in response to Her2 expressing cell lines using 3H incorporation assay ,,, First I need to stop proliferation of my cancer cells...
01 April 2017 7,283 2 View
