Ashmenta Robrams

10 Questions 7 Answers 0 Followers

Questions related from Ashmenta Robrams

Can I generate Dendritic cells from NSG mice?

Hi I'm trying to generate murine DCs from NSG HLA A2 mice ,,, using 20ng GM-CSF However, all what I get is MHCII neg, F4/80 hi, which show they are macrophages ,,, So I wonder can I generate...

27 March 2019 1,155 3 View

Can I differentiate between mouse M1 and M2 Macrophages in FACs using these Abs only?

Hi every one I wonder if I can differentiate between M1 and M2 macrophages in mouse tumours by using the following markers only? CD11b, CD11c, Ly6C, Ly6G, F4/80, MHCII If not ,,,which Ab I...

21 June 2018 7,291 2 View

How much time dendritic cells need to process adeno virus to be presented on their surface?

Hi I need to infect dendritic cells derived from mouse bone marrow with adenovirus then co culture them with T cells I'm wondering how much time the dendritic cells need to process the virus...

06 May 2018 9,631 2 View

What are the best markers for immature Dendritic cells derived from mouse bone marrow?

Hi I need a good panel for my FACS experiment which I 'll differentiate (mature DCs- which pulsed with peptide) and immature DCs ? Thanks

05 May 2018 2,128 3 View

Please help I need a good protocol for adenovirus titration???

Hi everyone I'm trying to titre some stocks of adenovirus that I isolated from infected 293a and I need a protocol to see the infection efficiency as the plaque forming assay However, I tried...

25 February 2018 7,055 3 View

How many nM that I give to my mice according to the following info?

Hi I give my mice 15mg/kg of a specific drug So how many nanomolar I give to my mice If the drug's formula weight is 439.52g/Mol ?? Thanks

28 January 2018 5,798 3 View

Pulsing mouse splenocytes with virus

Hello everyone I wonder how can I pulse my mouse splenocytes with virus to be presented on MHC for co-culture experiments how many particles is enough for pulsing ,,, 10 virus / 1 cell? 50virus...

26 July 2017 4,210 2 View

What the shape of cancer cells mean invivo?

Hi What does the different shape of cells mean, invivo subcuatneous, cells are from single sorting spindle shape and round shape of pancreatic cancer cell line from KPC mouse ? thanks

21 May 2017 2,975 1 View

Monocytic vs Granulocytic myeloid-derived suppressor cells (MDSCs)?

Is there any difference when I find Monocytic or Granulocytic myeloid-derived suppressor cells ( MDSCs ) in the tumours? Thanks

18 April 2017 2,130 1 View

What is the X ray irradiation dose to stop proliferation of cancer cell lines?

I need to test the proliferation ability of anti-Her2 CAR T cells in response to Her2 expressing cell lines using 3H incorporation assay ,,, First I need to stop proliferation of my cancer cells...

01 April 2017 7,252 2 View