Must I remove Triton X-100 and/or NaCl before before Bradford assay and/or SDS-PAGE (with BME)?

Hello! I'm purifying a protein and have several questions about what is appropriate to include in the extract solvent at different stages of the purification and characterization phases. (1)...

05 August 2020 4,625 7 View

AAV transduction of kupffer cells/macrophages in vivo?

Hi, We are looking to knock down a transcription factor in kupffer cells, preferably with AAV to allow cell type specific cre-dependent miRNA expression in lysMCre mice. Thus, we would...

05 June 2018 3,336 1 View

Can anyone help me, how to isolation DNA from fly (Lepoptena cervi) using Qiagen kit?

We are collected some deer ked fly. We want to go for molecular characterization of these flies. Kindly help us to extract DNA from these samples.

25 April 2018 8,088 3 View

What is the meaning of broad peak and sharp peak in Cyclic Voltameter (CV)?

I am working on electrochemical Oxidation of primary alcohols. For different catalyst the onset potential is same but the oxidation peak sharpness varies. I want to know what that means ?

29 January 2015 3,601 5 View

What are the effects of nuclear radiation on electronics?

Gamma rays can induce strap currents of electrons that generate harmful electromagnetic field similar to E M P (Electro-Magnetic Pulse). So, will the effect of radiation on the electronics be the...

05 December 2014 3,137 7 View

Is the FTIR spectral analysis method a perfect root to identified the organic and inorganic function groups?

In the case of surface-modified nanoparticles which contain both H2O and -OH groups, how can we distinguish -OH frequency?

29 October 2014 1,306 2 View

How can we switch on the IR Compensation and disable automatic current range in autolab cyclic voltammetry instrument (Nova Software)?

Handling this instrument is totally new for me and I can't reach to the solution to this problem. Please can anybody help me.....

14 October 2014 277 1 View

Are there any differences in the intensities of Nano level materials of the same compound with different shapes (sphere and rod)?

Formation of crystal shape is dependent on the particle's growth in an unique direction. Will the intensities differ?

10 October 2014 9,009 3 View

How do we confirm the actual crystal structure from XRD by matching with PCPDF file?

06 October 2014 1,537 5 View

How the wettability of rock sample can be changed by ageing in pure hydrocarbon oils only?

I want to do wettability analysis of hydrocarbon oil (octane)/rock/surfactant solution system. but to do the analysis first I have to make rock surface oil-wet. Since hydrocarbon oil does not have...

27 February 2021 5,383 3 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View

How can Gamma Ray API units be converted to CPS (counts per second)?

Oil and gas companies run gamma ray tool and take readings in API units while Gamma ray is also noted in cps, so how these units can be converted into each other?

24 February 2021 7,545 2 View

How to remove compound ( Europium complex ) from paraffin oil?

How can I remove my compound from paraffin oil? Mistakenly my compound fell in an oil. Compound = Europium complex

22 February 2021 5,667 2 View

How the values of errors can be calculated for thermodynamic data?

I have calculated thermodynamic parameters for surfactant solutions in aqueous and mixed alcoholic solvents. I want to calculate the value of errors in the data and represent it in the table along...

22 February 2021 239 2 View

When I mix oil and NaOH solution, Why solid layer in the surface is formed?

During my trial for lubricant oil re-refinery process, I use NaOH as coagulant agent. After overnight of gravity settling, a solid layer in the surface was form. what product is that? since the...

22 February 2021 2,116 4 View

The type of filter paper used to filter an epoxidized oil (the mixture is an epoxidized oil with magnesium sulfate) ?

I have a mixture of epoxidized oil and magnesium sulfate wich type of filtre paper should i use

21 February 2021 3,944 3 View

What reaction will occur when adding hexamine into ammonium nitrate in an acidic medium?

Hello, I am investigating properties of water in oil emulsion explosives. And adding hexamine (hexamethylenetetramine) into one of the phases that are used to make the emulsion should increase the...

21 February 2021 8,680 1 View

How can we study the adsorption efficiency of microgel at the oil-water interface?

I am studing the mircogel at oil-water interface. I want to know the adsorb amount of microgel at the oil water interface. How we can determine this amount?. I will highly appreciate your...

19 February 2021 2,787 1 View

Does anyone know, can i do cryosectioning of whole aorta after oil red O staining and use the cryosection parts for further analysis?

I would like to quantify the lesions on aorta. I want to take image of atherosclerotic plaques formation in aorta by using O red Oil Solution. After the staining, what will be the storage...

16 February 2021 5,876 2 View