Must I remove Triton X-100 and/or NaCl before before Bradford assay and/or SDS-PAGE (with BME)?

Hello! I'm purifying a protein and have several questions about what is appropriate to include in the extract solvent at different stages of the purification and characterization phases. (1)...

05 August 2020 4,625 7 View

How to determine the dislocation density of the deformed metal through XRD ?

suggest me some journals to learn about the technique to do and analysis.

21 September 2018 2,275 3 View

AAV transduction of kupffer cells/macrophages in vivo?

Hi, We are looking to knock down a transcription factor in kupffer cells, preferably with AAV to allow cell type specific cre-dependent miRNA expression in lysMCre mice. Thus, we would...

05 June 2018 3,336 1 View

What surfactants are best for an oil based magnitite ferrofluid?

I seeks something more effective than oleic acid. Ideally I would like something that is also non toxic in addition to being most effective*. *: Able to keep particles separated and have a low...

23 April 2016 1,435 3 View

What is the meaning of broad peak and sharp peak in Cyclic Voltameter (CV)?

I am working on electrochemical Oxidation of primary alcohols. For different catalyst the onset potential is same but the oxidation peak sharpness varies. I want to know what that means ?

29 January 2015 3,601 5 View

What are the effects of nuclear radiation on electronics?

Gamma rays can induce strap currents of electrons that generate harmful electromagnetic field similar to E M P (Electro-Magnetic Pulse). So, will the effect of radiation on the electronics be the...

05 December 2014 3,137 7 View

Is the FTIR spectral analysis method a perfect root to identified the organic and inorganic function groups?

In the case of surface-modified nanoparticles which contain both H2O and -OH groups, how can we distinguish -OH frequency?

29 October 2014 1,306 2 View

How can we switch on the IR Compensation and disable automatic current range in autolab cyclic voltammetry instrument (Nova Software)?

Handling this instrument is totally new for me and I can't reach to the solution to this problem. Please can anybody help me.....

14 October 2014 277 1 View

Are there any differences in the intensities of Nano level materials of the same compound with different shapes (sphere and rod)?

Formation of crystal shape is dependent on the particle's growth in an unique direction. Will the intensities differ?

10 October 2014 9,009 3 View

How do we confirm the actual crystal structure from XRD by matching with PCPDF file?

06 October 2014 1,537 5 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

12% ledeburitic cold work tool steel, etched for revealing retained austenite, any help for clarifying?

Hello everyone. Given microstructure image belongs to a 12% ledeburitic cold work tool steel (annealed and tempered). The sample was etched with picral (100 mL ethanol and 4 g picric acid). I...

02 March 2021 4,119 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View