I am working in non reducing condition (in reduction condition the gel is fine), and i assume my sample contains phospholipids, I remove the fat, change loading content 2-10 μl, voltages 150 to 200 V etc. and i did not obtain a good gel in non reducing condition.
Before trying anything out why not check the pH of your buffers - loading dye or running buffer? The smear thing is mostly associated with the fluctuating pH. Also the volt is too high which will also burn your protein. So try with 120V with between 4-20% gel concentration based on the size of the protein. I hope it helps, goodluck.
Giovanni,
Given the lack of details in your questions, I'm not surprised that you are getting amusing (or absurd) responses. ;)
Gels come in many sizes, either one must specify the length of the gel or provide a potential difference/unit length to have some meaning. Likewise, giving volume without concentration of protein, size of well and thickness of gel is virtually useless.
In other words, to receive useful suggestions, provide details.
If your sample has a high lipid content, try precipitating the protein using methanol/chloroform (doi:10.1016/0003-2697(84)90782-6) before electrophoresis.
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