Hi Lads,
Most of the methods used to extract lipid in whey protein ingredients involves chloroform-methanol-exhane-ether in different ratio (e.g., Folch).
I am struggling to find a suitable method to extract only polar, non-polar and both in one time with solvent without damage protein (e.g, use of ammonia in rose-gottlieb). I know with only non polar lipid, non polar solvent are the suitable option. but what about with polar and specially phospolipids fraction? any ideas or comments?
Giovanni
Dear Sir. Concerning your issue about the selective method to extract polar and non polar lipid in whey protein concentrate. A modified procedure for extraction of total lipids from whey protein concentrates was developed such that stable emulsion with extracting solvents was avoided and the solvent system remained monophasic. Nonlipid contaminants from the extract were removed using gel filtration instead of traditional aqueous washing to prevent any loss of polar lipids. The extraction of total lipids by the modified procedure was complete and comparable with a reference procedure. Traditional thin-layer chromatography is tedious and more qualitative than quantitative for lipid class separation. Total lipids were further separated into free fatty acids, phospholipids, cholesterol ester, triacylglycerol, cholesterol, diacylglycerol, and monoacylglycerol, using modified solid phase extraction procedure. Columns with 2 g amino propyl packing allowed separation of up to 80 mg of total lipids into lipid classes gravimetrically. The values for anhydrous milk fat for all lipid classes agreed with those in the literature. Separation of total lipids into lipid classes with solid phase extraction is easy, quantitative, and can also be performed on a preparative scale. I think the following below links may help you in your analysis:
https://link.springer.com/article/10.1007/BF02540976
https://www.dolcera.com/wiki/images/Polar_Lipid.pdf
https://www.hindawi.com/journals/jamc/2016/9827369/
Thanks
Dear Giovanni,
There are some techniques practiced in our lab for polar lipids, phospholipids/ lecithin extraction from marine resources. Hopefully, these may help you.
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