Hello everyone,
We are working on the osteoporosis. During this study we needs osteoblast cells. So, we are trying to isolate the the mesenchymal cells from adipose tissue. I want to know the concentration of colleganease used during the isolation steps of mesenchymal cells.If any one expert in same field, kindly suggest me specific protocol. I have curiosity to know the best method to isolate sufficient/healthy number of cells.
Thanking you
Hi, in my study we learned that collagen producing cells as fibroblasts are useful together with the hidroxyapatite that has has to so round collagen type 1 generated must be applied, mesenquimal cells we have never used before, thank you for your question.
Dear Mohammad Zafaryab This is an interesting study hope its useful.
Chapter Isolation of Murine Adipose-Derived Stromal/Stem Cells for A...
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