Hi, I am wondering if anyone has had luck replacing APEL2 media (Stemcell Technologies) with something else? We are trying to induce mesodermal cells from IPSC for a vascularized organoid protocol (following protocol by Sun et al., 2022).
I am currently writing a masters thesis on reasons for occupational stress in the early childhood classroom, and want to include a short survey assessing participants work-related stress levels. I...
24 February 2021 8,678 2 View
I am currently planning out my masters' thesis design and will be working with a large data set with variables that have been meseasured at two time points. Intially I thought of just using a...
21 January 2021 9,384 4 View
I have previously used Primer to output species accumulation curve results (including Chao, Jacknife and Bootstrap estimators), and from memory if I had 40 study sites, there would be 40 numbers...
23 December 2020 8,514 2 View
Hi all, I have been trying to figure out a way to calculate a priori sample size for a mixed anova. I have a study that uses 2 x 7 x 2. The last IV is within subjects. I appreciate any help....
23 September 2020 3,635 3 View
Hello everyone, Our lab is currently looking to purchase a plate reader and I was interested in seeing what opinions people have about some that they have used. Some of the other labs on...
07 September 2020 2,736 3 View
Pls help! I would like to do IF staining with 2 antibodies on a metaphase spread. But I dont have a cytospin! How to do it without cytospin? And im using methanol:acetic acid fixation.
16 August 2020 488 1 View
For an upcoming research project I will have audio files of interviews conducted in Spanish. I am looking for a company that can transcribe and translate these interviews - resulting in English...
28 June 2020 769 2 View
I have recently extracted genomic DNA from some museum bees for sequencing, and from first look there appears to be some pollen DNA in the extract. I've had a Google but can't find anything...
25 May 2020 8,423 2 View
I have been trying to look at Pyk2 phosphorylation in my cells (human monocyte derived macrophages) for about a year via Western Blot, and am having a massive amount of difficulty doing so. I have...
16 April 2020 6,662 0 View
I am conducting a meta-analysis for the association between TNBC and BRCA1 and BRCA2. Can anybody help me as to what 2x2 frequency table to use- standardised mean difference, correlation...
05 March 2020 4,992 3 View
We have a AAA class Newport solar simulator. We changed the Xenon Arc Lamp recently (XBO450W, Make:OSRAM) as the lifetime got over. However, the intensity is coming to be only 0.2 sun instead of 1...
14 February 2021 9,998 3 View
My question is regarding a western blot experiment that I am performing to observe pERK signaling by stimulation with VEGF ligand (VEGF165 and VEGF121) on MDAMB231 cells and SW620 cells (both...
30 January 2021 1,039 3 View
I am planning to do single-nuclei sequencing for brain organoids. However, the organoids experienced 25 minutes in room temperature prior to freezing. Most of the time they were in their culture...
17 January 2021 5,257 1 View
Mouse intestinal organoids ; After fixation and storage in PBS-T, when I checked in microscope, the organoid show fluorescent color blue even if I have not added DAPI. Does anyone have such...
05 January 2021 2,067 3 View
I am trying to find the cosine of sun elevation angle on slope terrain, I found the values between -0.9 to 0.9. Should the value be set to 0 - 0.9 or the negative value acceptable?
24 December 2020 4,027 4 View
I have an ordinary vascular system, where I know the inlet condition of pressure on the system and value of flowrate through each outlet. Based on these two pieces of information I need to...
16 December 2020 1,599 1 View
I work with ovarian cancer cell line organoids embedded in matrigel, they grow well the A2780 cells, but once I start using 10% formaldehyde to fix them they disintegrate...does anybody have a...
29 November 2020 1,900 1 View
There is too much discrepency among the textbooks of vertebrate embryology. There are two interpretations of the term "Prechordal plate" in different books: It is the cranial most part of the...
24 November 2020 8,537 1 View
Assume you have 1 cubic meter of water with 1 square meter of surface exposed to the sun. If you kept adding salt to the solution, is there a point at which evaporation would cease? Assuming the...
22 November 2020 5,444 1 View
I would like to get organoids of the same size. We filter organoids using a 100 um filter and then pass that filtrate through a 40 um filter followed by flipping the 40 um filter. Then we wash the...
22 November 2020 9,391 3 View