Hi, I am wondering if anyone has had luck replacing APEL2 media (Stemcell Technologies) with something else? We are trying to induce mesodermal cells from IPSC for a vascularized organoid protocol (following protocol by Sun et al., 2022).
I am culturing HEK293 cells. When I plated these cells in a 96-well plate, the cells looked healthy and there were no precipitates. However, after I dosed them with the drug RITA, they developed...
30 May 2024 5,169 2 View
Hi, if someone has data that are scored: 1 for poor, 2 for adequate and 3 for good. Can you take a mean average if a score of less than 1 is not possible? I'm thinking if a mean score of...
23 April 2024 6,366 1 View
I have a copy of a published paper that I want to spotlight on researchgate. What do I do? Thanx. Emily Kuriloff
09 April 2024 6,167 2 View
I am conducting a cell viability assay on a large sample of adherent cells unable to be distributed into a traditional sized 96 well plate. How would I analyze this larger sample? Would an ATP...
23 March 2024 8,249 1 View
I am testing cancer prevention mechanisms and am going to dose HEK cells with ethidium bromide to cause cancer. What is the procedure for dosing with ethidium bromide?
17 March 2024 8,415 1 View
Hello all, I recently started submitting neuroblastoma mouse tumor samples for single-cell RNA sequencing but am facing a huge problem with my tumor digestion being extremely gluey and slimy....
11 February 2024 6,603 0 View
I am doing ICC to check for colocalization of my protein of interest and mitochondria. Without having to use Mitotracker, (I'm currently have issues with finding the optimal concentration) or...
01 February 2024 3,815 1 View
I have a recently published paper receiving lots of reads, and would like to make it into a spotlight.
18 December 2023 1,668 2 View
I am running the CometAssay on cryopreserved avian blood samples. I had been obtaining what I thought were good results but then encountered a cloudiness problem the first time I tried to do more...
16 November 2023 8,530 0 View
Is it necessary to have a special microscope filter to visualize transient transferred mKeima cells? The microscope I have been using has the typical GFP and mCherry filters, but not a special...
18 October 2023 5,672 1 View
Do experiments on behalf of Our Business: Laboratory animal rearing Animal modeling Experimental animal behavior detection, image ultrasonic detection, electrophysiology platform Organoid...
05 August 2024 5,829 1 View
a second update to the checklust of the vaSCULAR FLORA NATIVE TO ITALY
24 July 2024 2,403 0 View
Is it feasible to employ an artificial sun in the experimental investigation of concentrated solar energy systems, specifically linear Fresnel systems? If the answer is affirmative, what type of...
22 July 2024 9,710 6 View
The earth has a orbit, but not for the sun. Similarly, all planets have orbits, but not for stars like the sun.
18 July 2024 1,768 4 View
I'm currently working with human pancreatic organoids and I am wondering to remove the dead cells and debris from health organoids, any suggestions would be appreciated!
17 July 2024 8,330 1 View
Hello everyone, I am cultivating patient derived organoids from pancreatic cancer patients and I saw at the bottom of my domes some "strange" cells other than my usual organoids. I know I should...
15 July 2024 762 3 View
"Surya Pradana asked What do you think about the shining?" is a thought-provoking question that encourages us to reflect on the concept of shining or radiance in various aspects of life. The idea...
11 July 2024 9,513 0 View
I'm currently working with mouse uterine organoids and I've seen that many authors perform organoid passaging using mechanical or enzymatic methods to dissolve the Matrigel while breaking the...
01 July 2024 381 2 View
The “intrinsic energy” of a particle is well known, it is mcc so what about of the “self energy” of a particle if one want to call it so and the “limit energy” of a particle if one want to call it...
24 June 2024 3,366 3 View
I'm working with intestinal organoids derived from children's polyps. I use Matrigel and Human IntestiCult growing medium, and sometimes I find these structures that resemble extracellular matrix...
23 June 2024 3,938 2 View