We are doing primary osteoblast culture from mouse calvaria, but we always get those very big cells as shown in the red cycle of the picture, which takes a lot of areas and blocks the real osteoblasts to grow. Does anyone know what is those cells and how to get rid of them from the beginning? Thanks a lot!
Tatiana Gritsaenko
I can't see them very well, but it looks like they have multiple nuclei. In this case they are osteoclasts (which would be logical, because you'll always have some monocytes precursors in the calvaria).
From my experience, they don't interfere with osteoblast amplification and they have a short life span - 5 days maximum.
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