What should be needle wash solvent for HPLC?

More Kartik R. Roy's questions See All

What are the fundamental ideas of Monte Carlo dimensionality estimation, compared to Rasch residuals PCA?

I read an interesting article from Christensen (2007): A Monte Carlo approach to unidimensionality testing in polytomous rasch models. But do you have some further thoughts or maybe other...

08 August 2024 4,245 0 View

How to quantify polystyrene microplastic (8 micron) bioaccumulation in fish tissue?

"I have treated adult zebrafish with 8-micron polystyrene microplastic and want to study the bioaccumulation in different organs. Can this be done using hydrogen peroxide digestion followed by...

05 August 2024 853 3 View

In the presence of VERY high concentration (1000 ppm) of silicon, the adsorption isotherms of arsenic are showing anomalous trends, why so?

I conducted an adsorption experiment of arsenic on soil in the presence of different doses of silicon as competing ions to see the effect of silicon on arsenic adsorption and desorption. I took 5...

03 August 2024 6,500 3 View

Flow through curved domains?

Hi everyone, I am working on a curved domain in which a ship is situated in the middle (geometry is given below). In my understanding the general fluid flow is parallel to the x axis from inlet to...

25 July 2024 9,058 4 View

I encountered a situation where the 16s PCR amplification worked perfectly with the DNA from the same sample, but using the same sample Don't working?

I isolated microbial DNA from marine water samples taken at various sites using the Qiagen Power Water DNA Isolation Kit, including a modified enzymatic lysis step. I encountered a situation where...

24 July 2024 9,688 0 View

What is the Principle of Cysteine-carbazole-sulfuric acid reaction with glucose?

I have been looking for the principle of Cysteine-carbazole-sulfuric acid reaction in research papers and books, but couldn't find it. This reaction is mainly used for the quantification of...

23 July 2024 3,891 0 View

Can Langmuir constant related to bonding energy (Kl) and Freundlich exponent (1/n) indicative of ads intensity show opposite trends with temperature?

The adsorbate is Arsenic and the adsorbent is a soil

18 July 2024 332 8 View

Reversed flow at outlet due to the release of DFBI?

Hi everyone, I am working on a simulation involving restricted canal with ship using DFBI. I am facing reversed flow in my outlet boundaries as the DFBI is released (In 1.25s). Is there any...

17 July 2024 7,032 1 View

Cloning of flaviviruses is difficult i have noticed that cells loose their transformation ability after cloning fragments inside vector any advice?

As flaviviruses genome is very frequently prone to mutation hence it is very difficult to clone whole genome inside vector what kind of vector and cells can sustain such amount of cloning...

11 July 2024 4,284 0 View

I am not getting proper colonies while ligating one of fragments in my vector the colonies seems to be normal but in light it seems lysed any reason?

I am working on cloning of a gene fragment inside my vector everything we tried to do correctly like making insert and vector with utmost care but after ligation the morphology of colonies seems...

11 July 2024 9,010 3 View

How to remove/ replace trifluoroacetate countertop after deprotection?

After deprotection of the tert-butyl ester to generate the carboxylic acid in chloroform with TFA, my compound‘s pyridine moiety forms a TFacetate ammonium salt (small molecule drug, not a...

29 July 2024 9,753 3 View

Why methanol and sulphuric acid used in the analysis of polyhydroxyalkanoates (PHA) by GC-MS?

Why methanol and sulphuric acid, used in the analysis of polyhydroxyalkanoates (PHA) methyl esters by GC-MS? Additionally, why do we typically use non-polar solvents in GC-MS?

22 July 2024 1,210 2 View

What solvent should I use to make a Schiff base from a ketone starting material?

I need to do a Schiff base reaction starting from a ketone and 1,10-phenanthroline-5-amino. I've seen that both of these starting materials are not very soluble in methanol and ethanol, which is...

23 June 2024 5,925 3 View

Hello.I have a starting material that has both carboxylic acid and alchohalic OH group.I added triphosgene to convert thoes groups to acid cholorid?

and chloroformate. But it takes 10 days to compete the reaction. How can I shorten the time of the reaction?

14 June 2024 2,924 3 View

Extraction RNA with miRVANA kit?

Hello everyone, I would like to know if any of you ever tried to assess the yeld of RNA extraction with the mirVana™ miRNA Isolation Kit, without phenol from ThermoFisher. I am experincing some...

06 June 2024 5,715 4 View

Separation of Cholesteryl esters from alkanes/alkenes?

Greetings We are trying to study Cholesteryl esters. We start with MeOH/Chloroform extraction and then remove most of the noise by silica column, but the Cholesteryl esters come out with...

02 June 2024 5,545 0 View

How to extract good quality RNA from Brown Algae?

We have been trying to extract RNA from brown algae. We have been following traditional CTAB method as modified by Sim et al., 2013. We get a very thick white jelly interface with the Chloroform...

30 May 2024 356 0 View

How can I salvage a failed trizol/chloroform RNA extraction using the first interphase?

I've been working off an RNA extraction protocol using trizol and chloroform for several months that worked beautifully until I moved on to our most important samples. After completing them, I...

23 May 2024 2,475 1 View

How to obtain a cleaner RNA solution?

Im extracting RNA from tomato leaves by the TRIZOL method but since the chloroform phase separation I can see that the aqueus phase is brownish and the pellet is totally brown. When I evaluate the...

21 May 2024 9,910 1 View

How to increase yield and purity of RNA from muscle of EEL using TRIzol??

For my project, I need to extract RNA from muscle tissue of EEL. I am trying but the yield is low and Purity not good. The 260:230 ratio is always below 1.50 and 260:280 ratio ranges 1.6-1.86 and...

15 May 2024 3,024 2 View

Samarth Zarad

chloroform is miscible with IPA and ethanol, you can use water: ethanol(50:50) or water :IPA (50:50)

Bruce Neagle

Your needle wash can vary and is usually 'stronger' than the target molecule and its sample matrix.

Avtar Singh

You should try a mixture of Methanol and Water.

Sony Ahmed

You should try a mixture of Methanol and Water (50:50) .

Salman Khurshid

try magic mixture (Water: ACN: IPA: MeOH) with a ratio of 1:1:1:1