Im extracting RNA from tomato leaves by the TRIZOL method but since the chloroform phase separation I can see that the aqueus phase is brownish and the pellet is totally brown. When I evaluate the RNA 260/230 rate is lower than 1. I have tried different methods such as CTAB at 1.5% in buffer, double chloroform phase separation, etc.
How can I improve this result??
Dheeravathu Venkateshwarlu
https://www.nature.com/articles/s41598-022-06810-7.
Try first two steps of trizol method (till chloroform), then use aqueous solution as a starting material in kit method (any kits u can use).
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