Kartik R. Roy

10 Questions 8 Answers 0 Followers

Questions related from Kartik R. Roy

What are dose and exposure time for cancer radiation therapy? Please, give some ideas or papers. Thanks?

Cancer radiation therapy.

12 September 2018 7,787 5 View

Why double band appeared on TLC plate?

I run standard mixture on TLC plate. All standards showed with double band.

27 February 2018 3,436 3 View

What is the differences between gene silencing by shRNA and miRNA? What are procedural and theoretical differences?

I want to know advantages and disadvantages of gene silencing by shRNA and miRNA?

11 February 2018 9,030 3 View

How much sample (in amount) should be loaded on TLC plate?

I am loading 10 microliter sample slution on TLC plate. I want to know the amount of sample should be loaded on plated for detection. Thanks.

27 January 2018 4,871 6 View

How can I download protein structure having mutation hotspot mark?

I want to download p53 protein structure with hotspot mutation mark from protein data bank. But I could not. Would you suggest me how to use PDB or other website to see protein structure? Thanks

22 January 2018 9,769 1 View

How can I determine lipid raft presence in sucrose gradient ultracentrifugation?

I am isolating lipid raft from animal tissue and cancer cell. How can I determine layer/layers in which lipid raft is present? I am using 5%, 40% and 80% sucrose gradient ultracentrifugation.

07 January 2018 4,085 4 View

Could you tell me details about sphingolipid extraction from cell? What factors should be optimized for extraction?

I am working on sphingolipid extraction from cell. I am using chloroform, methanol and water at same ratio for extraction.

12 July 2017 5,387 2 View

What should be needle wash solvent for HPLC?

I am working on HPLC in which solvent system contains Chloroform, methanol, water and orthophosphoric acid. I want to avoid left over contamination. What should be needle wash solvent in this case.

27 June 2017 3,123 5 View

How I can extract membrane protein (E-cadherin and Fibronectin) from cell (human cancer cell) by using NP-40 lysis buffer?

I am using NP-40 lysis buffer to extract membrane proteins. Last two attempts I did not get these proteins. How can I use this detergent containing buffer to extract membrane proteins. Thanks

05 June 2017 206 4 View

Anyone working on drug resistance of TP53 cell line?

How many passages usually need to develop drug resistance for this cell line by two drugs? Could you let me know the cell response to these drugs? 1. Cisplatin 2. 5-FU

23 November 2016 7,567 2 View