In the course of bioprinting the need in temporal (sacrificial) material arises very often. The choice of the material could be based upon:
- Main biomaterial properties and, thus, printing conditions (e.g. temperature);
- Printing accuracy required in an experiment;
- The cost.
Among the materials: gelatin (with low or high Bloom value), Pluronic F-127, PEO (polyethylene oxide), and etc.
What material is the first option in your studies and why?
P.S.
We partly discuss the issue. But there are still a lot of options.
https://www.researchgate.net/publication/350828575_The_Technique_of_Thyroid_Cartilage_Scaffold_Support_Formation_for_Extrusion-Based_Bioprinting
There are two methods. In the first case, they use materials to make support layers that are embedded in the body. This experiment can be by heat or chemical. Decomposition materials should not be harmful to the body and the amount of decomposition should not be so high as to cause problems for the body. The second method I prefer is to be able to remove the support layers from the printed object before inserting it into the body.
Dear Abbas,
Thank you for the reply. But what exact material do you usually use for the purpose?
Use Alginate. As it has many advantages like fast gelation, better mechanical strength, bioinnert, biocompatible, easily available, low cost, favourable physicochemical conditions for cell proliferation and cell growth. Alginate Bioinks can be tuned easily with other polymers for improving the erratic degredation and inadequate degredation.
Dear Sudipto Datta
The question is not about bioink proper but is related to the addition hydrogel that should be used in the case of complex inner structure or (external) geometry of a scaffold.
You can use gelatin, collagen etc. In my PhD work I mixed honey with alginate to improve the biocompatibility without affecting the physicochemical, mechanical and shape fidality too much. I used antioxidant NAC to improve biocompatibility and cell material interaction as well as cell apoptosis. You can also use poly amino acids with alginate.
Sudipto Datta ,
I think there is some misunderstanding here. Hydrogels like collagen are used as cell-laden materials. Sometimes, in the case of extrusion-based bioprinting, an additional material might be required for temporal support of the scaffold or to add hollow channels and pores to the scaffold. These materials must be water-soluble like unmodified gelatin. Do you have such an experience?
Use honey, ployaminoacids, N-acetyl-cyctine I used these with alginate for bioprinting in my research work.
Sudipto Datta,
Could you give me links to a couple of your articles?
https://link.springer.com/article/10.1557/jmr.2018.202
https://www.tandfonline.com/doi/abs/10.1080/00914037.2018.1539988?journalCode=gpom20
https://www.sciencedirect.com/science/article/pii/S2452199X2030075X
https://pubmed.ncbi.nlm.nih.gov/31610661/
use low concentration gelatin and drop the temperature then its washed away. Another method is to use poloxamer 407 (pluronic 127). dissolve in the cold temperature, printing in room temperature, drop the temperature then it will be removed by itself.
Mohamadhasan Ansarizadeh,
Thank you for your answer.
I think you meant in the case of gelatin - raise the temperature...
Pluronic is a unique material regarding state transition.
Any other options except these two?
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