Is there no general protocol for vaccination study?

Hi, I searched vaccination study to know which MLD50 is suitable for influenza virus study in pubmed. But why every vaccination and challenge conditions are different? Is there no guideline or...

16 January 2021 7,984 3 View

Plaxis V20 water load apply error in analyses?

Recently I have been working in some embankment project and plaxis V20 is the FEM software I am using to calculate. But by the time I am working, I figured out the water load sometimes applied to...

27 October 2020 7,835 1 View

Is there any mathematical way to represent any function on a z=0 plane transform into an arbitrary differentiable continuous non-linear plane?

Dear scholars, I am now struggling on a question. Let's assume that there is a given line or a given arbitrary function defined on a z=0 plane. Now I twist the plane into a non-linear 3D surface...

03 June 2020 3,693 1 View

Is it OK culture E.coli at RT for overnight?

For plasmid DNA isolation, E.coli was incubated last night. But incubator doesn't work and I just knew it this morning. The incubator fixed now. Is it okay for my experiment?

28 April 2020 2,931 10 View

Where can I get a reliable list of Wieser's (1953) nematode feeding type classification?

Numerous articles cite it but nobody seems to provide an actual list of the taxa and which of teh four groups they are assigned to.

21 March 2020 5,990 5 View

What solvent should I use with SPE to elute amines from silica?

Hello, I am extracting polar compounds from organic samples using SPE. After loading the sample, I use pure methanol to elute the polars. However, I believe alkyl amines are being retained on the...

26 September 2019 854 3 View

Do you have the matlab code of linear canonical transforms ?

I want some MATLAB code about linear canonical transforms in signal processing. Because I research this problem for my subject, I want to know basic way by MATLAB code. This is my first time to...

13 September 2019 9,746 3 View

Acquisition and tracking of BOC signal in multipath environment?

I want some MATLAB code about acquisition and tracking of BOC signals. Because I research this problem for my subject, I want to know basic way by MATLAB code. This is my first time to get this...

15 August 2019 652 2 View

Dear all, I have a question concerning executive function. What does Executive Function inlcude? What tools can be used to assess EF?

My study is going to compare ASD and TD group on their performance in different linguistic items. Do I need to match them on EF? Why or why not?

04 July 2019 8,770 3 View

Progress in integrated utilization of solar energy (Especially for sorption technologies)?

Utilization of solar energy is always one of the meaningful research areas. But most researches are about one or two utilization aspects, such as for cooling, heating, heat storage, power...

03 June 2019 6,983 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

What software programs can directly simulate Additive Manufacturing Directed Energy Deposition (DED)?

I understand there are a lot of software programs that can simulate DED by going through specific modeling, simulation, and commands. For me, that was time consuming and results were not accurate....

24 February 2021 4,428 3 View

How to dissolve cross-linked Amberlite IRC120 H, hydrogen form resin?

Hello all, I have used Amberlite IRC120 H, hydrogen form acidic cation-exchange resin for the ion-exchange of waterglass [sodium silicate solution -- 40% Sodium silicate (mol ratio > 3.2)...

21 February 2021 5,614 3 View

Best protocol for immunogold using araldite resin ?

Hello, We perfom Tokuyasu method (ultrathin cryo sectionning) very often to make immunogold experiments. This time, we need to make a PLT technique with K4M or LR white resin. Our results were...

14 February 2021 4,959 1 View

How to improve the adhesion of epoxy coating on steel substrate? Which factor affects?

I am facing a problem regarding a de-adhesion problem with the coating. A brief description of my methodology is given below. I am using mild or Low Carbon steel as a substrate and I polish the...

14 February 2021 2,167 4 View

2D wavelet transform?

Dear all I have a 2-dimensional mode shape of one concrete beam and I put the data of mode shape in a matrix (69*3) which the first column in the matrix is x coordination of my beam and the second...

10 February 2021 3,301 3 View

Purified protein requires Calcium for activation, but the elution buffer I used seems to interact with Calcium, help?

09 February 2021 6,946 4 View

FLAG purification, elution doesn't have protein?

I am purifying a heterotetramer, with one subunit having a 1D4 tag and the other having a FLAG tag. I purified from Sf9 with HBS pH7.3 and 0.002%LMNG first with Anti-1D4 resin and got an adequate...

02 February 2021 3,860 1 View