What protein if fluorophore-tagged, will mark all skin epithelial keratocytes in adult zebrafish and not just the superficial epithelial cell layer?

07 December 2022 0 5K Report

I know of the skinbow construct that would barcode each cell in the SEC layer of zebrafish epithelium, but I want to mark all the skin epithelial keratocytes to be able to follow them after creating a wound. What protein, in this case, would serve as the best marker?

Badges
Science topic

More Alekhya Hati's questions See All

Is there anyone working with refrigerants please add me to the groups?

i am a budding research scholar working with refrigerants and if there is anyone working in same area please contact me. it will help us better.

30 March 2024 4,227 2 View

Does anyone know how to calculate the vapour pressure of a refrigerant. please share some useful information to proceed further for pxy,txy diagrams?

does anyone know how to calculate the vapour pressure of a refrigerant? please share some useful information to proceed further for pxy,txy diagrams

22 January 2024 5,938 4 View

About Microscale Thermophoresis ???

Which software I can used for this kind of graph making other than affinity analysis softwere?????

07 December 2023 4,514 1 View

Can anyone tell me which HPLC column i should use for SEC of one 20-30kDa nonpolar compound??

Guys I have a compound which is 20-30 Kda and also nonpolar in nature. Any specific HPLC column anyone can suggest for purifying that compund????

22 July 2023 6,392 0 View

Which protein can be targeted as a marker for cells that possess lamellipodia?

In a wound healing experiment, I have multiple layers of cells in the sheet of cells. Only the cells in the lowermost layer of this cell sheet possess lamellipodium, while those on a higher plane...

25 June 2023 1,447 0 View

About Microscale Thermophoresis?

Can you please tell me what should be the range of response amplitude, kd confidence, standard error, reduced x2 and signal to noise ratio in Microscale Thermophoresis???????

07 June 2023 3,961 2 View

Is it possible to mount coverslips directly in a 35mm plastic glass-bottom dish?

In our lab, we use 35 mm plastic dishes with a 10 mm hole in the bottom, where we attach 22x22 mm coverslips using silica glue. We use this to grow cells and use it as the imaging dish for live...

01 May 2023 3,714 0 View

Are solutions of 3-(Trimethoxysilyl)propyl methacrylate (TMSPM) in isopropanol stable and reusable?

For an experiment, I need to make the surface of glass coverslips reactive. I would treat multiple coverslips put on a rack dipped in the solution of TMSPM. Since a large volume of solution...

11 April 2023 5,017 1 View

What alternatives can one use to BrdU staining to detect proliferating cells?

I culture multilayered cells, and I found previously that the live nuclear staining dye Hoechst 33342 enters the cells not in contact with the culture medium (cells in the lower layer) very...

18 December 2022 9,314 4 View

Can cells be separated from each other in an intact tissue after paraformaldehyde fixation of the tissue??

If, in a culture dish, cells form cell-cell adhesion junctions (as in an intact tissue), can they be separated into single cells after the fixation is performed with the junctions intact? I...

02 December 2022 1,925 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to quantify polystyrene microplastic (8 micron) bioaccumulation in fish tissue?

"I have treated adult zebrafish with 8-micron polystyrene microplastic and want to study the bioaccumulation in different organs. Can this be done using hydrogen peroxide digestion followed by...

05 August 2024 853 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View