If, in a culture dish, cells form cell-cell adhesion junctions (as in an intact tissue), can they be separated into single cells after the fixation is performed with the junctions intact? I understand that fixation cross-links proteins, which would act on the adherans-junction proteins also. I want to perform FACS on these cells. I do not want to trypsinize live cells as that might change the mechanical signaling state in the cells.