Guys I have a compound which is 20-30 Kda and also nonpolar in nature. Any specific HPLC column anyone can suggest for purifying that compund????
Which software I can used for this kind of graph making other than affinity analysis softwere?????
07 December 2023 4,514 1 View
In a wound healing experiment, I have multiple layers of cells in the sheet of cells. Only the cells in the lowermost layer of this cell sheet possess lamellipodium, while those on a higher plane...
25 June 2023 1,447 0 View
Can you please tell me what should be the range of response amplitude, kd confidence, standard error, reduced x2 and signal to noise ratio in Microscale Thermophoresis???????
07 June 2023 3,961 2 View
In our lab, we use 35 mm plastic dishes with a 10 mm hole in the bottom, where we attach 22x22 mm coverslips using silica glue. We use this to grow cells and use it as the imaging dish for live...
01 May 2023 3,714 0 View
For an experiment, I need to make the surface of glass coverslips reactive. I would treat multiple coverslips put on a rack dipped in the solution of TMSPM. Since a large volume of solution...
11 April 2023 5,017 1 View
I culture multilayered cells, and I found previously that the live nuclear staining dye Hoechst 33342 enters the cells not in contact with the culture medium (cells in the lower layer) very...
18 December 2022 9,314 4 View
I know of the skinbow construct that would barcode each cell in the SEC layer of zebrafish epithelium, but I want to mark all the skin epithelial keratocytes to be able to follow them after...
06 December 2022 5,453 0 View
If, in a culture dish, cells form cell-cell adhesion junctions (as in an intact tissue), can they be separated into single cells after the fixation is performed with the junctions intact? I...
02 December 2022 1,925 0 View
I am trying to study the migration of primary cells from explants and hence take long time-lapse movies. Every time I experiment, I need to take an explant and put it in the culture dish and wait...
29 November 2022 3,411 2 View
I routinely take skin explants and add to culture medium. Within 6 hours, sheets of cells migrate from the explant. I am able to perform staining on this without causing detachment of the entire...
23 November 2022 6,227 4 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Are there any suggestions or insights you can provide to help address this problem
06 August 2024 1,443 2 View
I am trying to recover liquid CO2 from a mixture of 0.6 CO2, 0.3 N2, and 0.1 O2. My aim is to recover about 99% liquid CO2 at the bottom of the column and make sure the amount of gaseous CO2...
06 August 2024 4,611 0 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
Mobile apps can be a powerful tool for enhancing academic performance, how can we use mobile apps for improving academic performance
04 August 2024 9,492 0 View