Can you please tell me what should be the range of response amplitude, kd confidence, standard error, reduced x2 and signal to noise ratio in Microscale Thermophoresis???????
A good response amplitude and signal to noise ratio depend upon the choice of ON-time or "T-hot" window selected for the analysis. At lower time intervals, amplitude can be as less as 1, but the S/N ratio needs to be above 10. As we move to the right and towards the higher time intervals, a good amplitude would need to be higher and S/N ratio can come down as low as up to 5. There is a table for the same in Nanopedia. Kd confidence needs to be within 60% of the Kd value calculated and would show a good fit. Reduced x2 should be around 1. Standard error ideally should be as less as possible. It depends upon how many points of the dose-response have been considered for estimation of Kd. However if the Kd confidence is good along with S/N ratio and amplitude, then you would observe the other two parameters take care of themselves. These parameters essentially help us to figure out the best "ON-time" to be considered for analysis, thereby helping us choose the most accurate Kd values.
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