Alekhya Hati

10 Questions 4 Answers 0 Followers

Questions related from Alekhya Hati

Which protein can be targeted as a marker for cells that possess lamellipodia?

In a wound healing experiment, I have multiple layers of cells in the sheet of cells. Only the cells in the lowermost layer of this cell sheet possess lamellipodium, while those on a higher plane...

26 June 2023 1,441 0 View

Is it possible to mount coverslips directly in a 35mm plastic glass-bottom dish?

In our lab, we use 35 mm plastic dishes with a 10 mm hole in the bottom, where we attach 22x22 mm coverslips using silica glue. We use this to grow cells and use it as the imaging dish for live...

02 May 2023 3,710 0 View

Are solutions of 3-(Trimethoxysilyl)propyl methacrylate (TMSPM) in isopropanol stable and reusable?

For an experiment, I need to make the surface of glass coverslips reactive. I would treat multiple coverslips put on a rack dipped in the solution of TMSPM. Since a large volume of solution...

12 April 2023 5,010 1 View

What alternatives can one use to BrdU staining to detect proliferating cells?

I culture multilayered cells, and I found previously that the live nuclear staining dye Hoechst 33342 enters the cells not in contact with the culture medium (cells in the lower layer) very...

19 December 2022 9,305 4 View

What protein if fluorophore-tagged, will mark all skin epithelial keratocytes in adult zebrafish and not just the superficial epithelial cell layer?

I know of the skinbow construct that would barcode each cell in the SEC layer of zebrafish epithelium, but I want to mark all the skin epithelial keratocytes to be able to follow them after...

07 December 2022 5,449 0 View

Can cells be separated from each other in an intact tissue after paraformaldehyde fixation of the tissue??

If, in a culture dish, cells form cell-cell adhesion junctions (as in an intact tissue), can they be separated into single cells after the fixation is performed with the junctions intact? I...

03 December 2022 1,921 0 View

Experiment design: how do I design appropriate experiment where the control and the drug treatment part are temporally separated by a day?

I am trying to study the migration of primary cells from explants and hence take long time-lapse movies. Every time I experiment, I need to take an explant and put it in the culture dish and wait...

30 November 2022 3,406 2 View

How do I prevent detachment of cell monolayer/bilayer while performing immunofluorescence staining in explants?

I routinely take skin explants and add to culture medium. Within 6 hours, sheets of cells migrate from the explant. I am able to perform staining on this without causing detachment of the entire...

24 November 2022 6,224 4 View

How do I acutely deplete cholesterol from primary cells in a medium containing Fetal Bovine Serum?

I am trying to study the collective migration of primary cells and to maintain cell-cell contacts and prevent fragmentation of the expanding tissue, I need to keep the cells in a medium containing...

26 August 2022 3,178 0 View

What is the best way to perform immunofluorescence staining without dislodging tissue from culture dish?

I work with primary cells (explant culture) and while performing IF staining, I require several washing steps. As the culture is a tissue, it seems to get dislodged by the time I perform all the...

17 March 2022 5,158 3 View