Relation betwen tool and plate in frw process

09 February 2017 1,841 2 View

Has anyone immunized mice with Influenza HA1 antigen?

I would appreciate if anyone can share information about HA1 immunizations in mice. Particularly Cal09 HA1 in C57BL/6 mice or BALB/c mice. Any information about the murine MHC class2 restriction...

07 June 2016 3,665 3 View

What is the best way to identify protein in FPLC fractions?

I subject NiNTA purified proteins to size exclusion chromatography to get rid of any impurities and identify monomers. I'm not entirely sure what the best approach is for further downstream...

01 March 2016 8,761 5 View

What are the advantages of detecting memory B cells using ELISPOT instead of flow cytometry?

I plan to do an immunization and look at memory B cells among other B cell subsets. I will be doing a flow cytometric analysis (I will use cell surface markers and also look at antigen binding...

26 September 2015 7,402 3 View

Does anyone have experience looking at t follicular helper cells and b cells using flow cytometry?

I stained mouse lymph nodes with pd-1,cxcr5,cd4 and cd44 for t follicular helper cells and cd19,cd38,cd73 and af488 conjugated to an antigen that I originally immunized the mice with. I collected...

22 March 2015 6,336 1 View

Why is capture ELISA assay not detecting not working or capture HIS-tagged GP120?

21 March 2015 7,090 4 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

What are the main reasons to grow Escherichia coli O157:H7 and Salmonella Typhimurium in food products under refrigeration storage 4 C?

What are the main reasons to grow Escherichia coli O157:H7 and Salmonella Typhimurium in food products under refrigeration storage 4 C?

24 January 2021 9,987 3 View

I am trying to simulate phase change of refrigerant inside pipe extracting heat from panel in comsol. Can anyone help with how to simulate this?

My model is a rectangular multi layered structure under which refrigerant flows through cylindrical pipes. A heat flux of 600W/m2 applied on panel and internal forced convection is used for...

20 January 2021 9,478 1 View

Is it possible to calculate entalphy of LiBr/H2O or another solution if we know correlation of specific heat without using any software?

Is it possible to calculate entalphy of LiBr/H2O or another solution if we know correlation of specific heat without using any software?

17 January 2021 5,522 3 View

How do i calculate the total heat transfer rate in COMSOL (5.4)?

I have modelled a condenser of a refrigerator in COMSOL. I made different designs and i want to calculate which design has the highest heat transfer rate. The way i calculate it now is by: q =...

02 December 2020 306 2 View

How can I determine the mass of a refrigerant (for example R744) in a evaporator when the refrigeration system is not working?

By knowing the specific volume on the inlet and on the outlet, is it correct to assume an average of those two specific volumes?

30 November 2020 3,807 3 View

Potassium formate/water or potassium formate+LiBr/Water are not available in the commercial sector for absorption refrigeration systems?

Although there are already studies in the literature but not much , the use of potassium formate/water or potassium formate+LiBr/Water are not available in the commercial sector for absorption...

16 November 2020 2,917 2 View

Is there any way to revive strains after a year stored in 4 degree refrigerator?

The strain is Candida tropicalis. It has been stored in 4 degree refrigerator for a year. Now we want to see if it is possible to revive the strain and make it function again. I tried to culture...

10 November 2020 5,650 9 View

No title

When producing a recombinant protein, harvested E. coli cells are normally refrigerated for 12-24 hours before homogenization to ensure protein inactivation due to foaming does not occur....

16 October 2020 7,373 0 View

How to calculate the dimensions and frequency, etc. of a pulse tube for required cold/hot temperatures?

Require to know the methodology for calculating dimensions (Lenght, diameter), operating frequency, etc. of a simple pulse tube refrigerator. Reference to handbook or manuscript would also be helpful.

29 September 2020 2,191 2 View