Is there anyone working with refrigerants please add me to the groups?

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Does anyone know how to calculate the vapour pressure of a refrigerant. please share some useful information to proceed further for pxy,txy diagrams?

does anyone know how to calculate the vapour pressure of a refrigerant? please share some useful information to proceed further for pxy,txy diagrams

22 January 2024 5,938 4 View

Which protein can be targeted as a marker for cells that possess lamellipodia?

In a wound healing experiment, I have multiple layers of cells in the sheet of cells. Only the cells in the lowermost layer of this cell sheet possess lamellipodium, while those on a higher plane...

25 June 2023 1,447 0 View

Is it possible to mount coverslips directly in a 35mm plastic glass-bottom dish?

In our lab, we use 35 mm plastic dishes with a 10 mm hole in the bottom, where we attach 22x22 mm coverslips using silica glue. We use this to grow cells and use it as the imaging dish for live...

01 May 2023 3,714 0 View

Are solutions of 3-(Trimethoxysilyl)propyl methacrylate (TMSPM) in isopropanol stable and reusable?

For an experiment, I need to make the surface of glass coverslips reactive. I would treat multiple coverslips put on a rack dipped in the solution of TMSPM. Since a large volume of solution...

11 April 2023 5,017 1 View

What alternatives can one use to BrdU staining to detect proliferating cells?

I culture multilayered cells, and I found previously that the live nuclear staining dye Hoechst 33342 enters the cells not in contact with the culture medium (cells in the lower layer) very...

18 December 2022 9,314 4 View

What protein if fluorophore-tagged, will mark all skin epithelial keratocytes in adult zebrafish and not just the superficial epithelial cell layer?

I know of the skinbow construct that would barcode each cell in the SEC layer of zebrafish epithelium, but I want to mark all the skin epithelial keratocytes to be able to follow them after...

06 December 2022 5,453 0 View

Can cells be separated from each other in an intact tissue after paraformaldehyde fixation of the tissue??

If, in a culture dish, cells form cell-cell adhesion junctions (as in an intact tissue), can they be separated into single cells after the fixation is performed with the junctions intact? I...

02 December 2022 1,925 0 View

Experiment design: how do I design appropriate experiment where the control and the drug treatment part are temporally separated by a day?

I am trying to study the migration of primary cells from explants and hence take long time-lapse movies. Every time I experiment, I need to take an explant and put it in the culture dish and wait...

29 November 2022 3,411 2 View

How do I prevent detachment of cell monolayer/bilayer while performing immunofluorescence staining in explants?

I routinely take skin explants and add to culture medium. Within 6 hours, sheets of cells migrate from the explant. I am able to perform staining on this without causing detachment of the entire...

23 November 2022 6,227 4 View

How do I acutely deplete cholesterol from primary cells in a medium containing Fetal Bovine Serum?

I am trying to study the collective migration of primary cells and to maintain cell-cell contacts and prevent fragmentation of the expanding tissue, I need to keep the cells in a medium containing...

25 August 2022 3,188 0 View

How to make overlapping p-h diagram in EES?

I want to draw ph diagram using Engineering equation solver EES for refrigerant R134a and R1234fy and some other. I don't know how to draw the multiple ph diagram on same dome or overlapping to...

30 July 2024 647 7 View

Why does the aloe vera gel change color from white to pink when placed in the refrigerator?

While when placed in the freezer, it maintains the same original white color?

05 June 2024 5,231 5 View

How to conduct comparative study of refrigerants with one heat pump model as they have different saturation pressure and temperature?

I have modelled a refrigeration system using finned and tube heat exchanger and adopted the tube by tube method. I chose the R134a as a baseline refrigerant and developed the model according to...

30 May 2024 8,152 12 View

Why would my comet assay of zebrafish blood have a cloudy appearance?

I did comet assay with goldfish in my master's degree but now I am experiencing such a problem. has anyone encountered such a problem before? the only difference from my other experiments is that...

09 May 2024 6,902 3 View

How many times do we refill R290 cold storage systems per year, what is the leakage frequency of propane natural refrigerant?

Hello, I need the reference that shows the leakage rate or the refilling frequency of R290 refrigerant. Thank you

17 April 2024 5,298 0 View

How to model or program a cascade refrigeration system using natural refrigerants on MATLAB ?

What are different ways of modelling and simulation of cascade refrigeration cycleusing natural refrigerants on MATLAB without using simscape ? How to write program for it and connect MATLAB...

02 April 2024 487 2 View

Knowledge graph of cold chain?

Dear Friends I hope you are well. "I'm interested in the concept of a 'Knowledge Graph of the Cold Chain.' If you have any files or information on this topic, could you please share it with me?...

05 March 2024 2,305 2 View

What is the stability of 1% N-(1-naphthyl)ethylene-1,2-diamine dihydrochloride (NEDA) solution?

I am measuring primary aromatic amines by UV-VIS and using the NEDA solution as my coupling reagent. The method says that the reagent needs to be made fresh every day. However, the solution is...

29 February 2024 6,575 2 View

How to detect noise sources from a home appliance?

for example: I am using a refrigerator for 3 years , recenly, 2/3 months ago the noise level seem increased. I made a complain to the refrigertor manufacturing brand. Now, how the R&D team of...

27 February 2024 6,870 2 View

How long can live tissue survive (in refrigeration 4℃) once removed from body?

I'm currently working with animal tissue, more specifically visceral fat tissues from dogs. Our labs are not equipped to function 24/7 at the moment so I have to make sure the sample collection...

25 February 2024 2,835 4 View