What Positive & Negative control is required for G2A Knockdown & Overexpression?

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How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

Is there any effect of different colours of light (white, red, yellow, blue etc.) on the rate of photosynthesis? If yes, why?

Light is inevitable for the process of photosynthesis reaction. Naturally sunlight plays the vital role for this process. Light may be of different colours like white, red, yellow, blue etc....

02 March 2021 7,890 2 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

What is the difference between L1 and L2 regularization in machine learning?

L1 and L2 regularization

28 February 2021 4,187 3 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

Hi i want to ask that in order to find out LC 50 (96 hours) of particular toxin for fish. Should we keep the animals with food or without food?

if we expose the fish after acclimatization to particular toxin in order to find out LC 50 (96 hours).Should we keep the fish off feed or with feed for that duration. Some literature show that...

24 February 2021 357 2 View

Can anyone suggest me the best way for Regression analysis of Accidents?

Dear Altruist, I want to make a regression analysis based on accident data. But I am not finding the necessary information about what type of regression is more applicable for analysis accident...

23 February 2021 9,956 3 View

How much cell pressure and back pressure should I apply to start for triaxial test for different types of soil?

We have recently purchased a triaxial test instrument (MATEST) for testing different types of soil. After installing software, we are facing a issue of saturation where the samples does not...

23 February 2021 7,194 4 View

How to assure whether a regression tree overfit or not by seeing bias-variance value of the model?

Lets consider a regression tree in which variance is 1.1065*e-10 and bias is 2.962e-13. Also the model RMSE on training set is 1.5e-5 and on training set is 1.2950e-5. Is the model is overfit or...

22 February 2021 7,755 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

It is easier to knockdown a gene than overexpress a gene when generate a stable cell line?

I am just so confused that some people tell me knockdown is easier than overexpression when generating a stable cell line. I think it depends on the gene. Cells won't be happy whether we try to...

15 February 2021 1,370 2 View

Would using an asRNA for repressing transcription of a high expression Listeria monocytogenes non-coding RNA harbored in a plasmid be a viable option?

Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...

09 February 2021 4,994 1 View

Should I add a promoter upstream of my CRISPR-inserted gene?

Hello, I want to insert a gene into my cells using sgRNA, Cas9, and an HDR template. The gene that I will insert into has its own promoter. Because I will cut the gene downstream of its start...

14 January 2021 1,170 2 View

Could you please advise me that any suitable techniques for this experiment which will be done shortly?

I am planning to perform the functional study of gene whuch is supplied maternally, so morpholino-mediated knockdown assay won't work. Also about the CRISPR mediated knockout study need to wait...

27 December 2020 731 1 View

What are the effects of longer exposure to siRNA (for KD)?

1) I knocked down a protein and collected cells 24 h and 72 h after transfection with Lipofectamine (to note, 5 h after transfection medium was replaced with medium with FBS). 2) KD efficiency...

08 December 2020 9,850 1 View

How does one induce cell death using CRISP-Cas9?

Hello, I want to target a specific gene sequence using CRISPR-Cas9 to induce the death of the cells carrying that gene. I think that there are three basic approaches: 1.) Induce a frameshift...

20 November 2020 4,617 3 View

Can someone let me know the best SiRNA along with negative controls for silencing NEAT1 in human breast cancer cells. Company and price if possible?

SiRNA for NEAT1 knockdown in breast cancer cells

05 October 2020 9,723 1 View

Ideas for knocking down protein that is heavily upregulated in cancer?

Hello all, I am trying to knock down a protein that has been documented to be heavily upregulated in oral squamous cell carcinoma (confirmed via western blot). I have attempted to knock down this...

31 August 2020 9,214 3 View

Can my RNA interference have any influence in my endougenos control gene?

Good night all. I'm using RNAi to knockdown a transcription factor from a primary cell and I need to validate by westernblot. With Ponceau marking i can see that i'm putting the same amount of...

25 August 2020 263 3 View