What Positive & Negative control is required for G2A Knockdown & Overexpression?

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Can we mark 'EFL Learners shifting from general digital to AI technologies' as technological transition?

After COVID-19 it has seen that EFL learners technological affiliation has raised. In addition, in the post-COVID period learners started to engage AI technologies like ChatGPT while learning...

08 August 2024 8,964 4 View

How to fix background error in rietveld refinement of one XRD peak using GSAS-II?

I want to refine one XRD peak of my in-situ xrd but the background is never working good which ultimately fails the refinement. How to refine and adjust the background using GSAS-II

05 August 2024 5,291 2 View

How to convert g/kg Humic acid dose to kg/ha?

I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?

02 August 2024 7,186 6 View

Bangladesh government's reported plan to use lethal force against protesters? We need help Urgently ?

Please Help Us Urgently

01 August 2024 711 1 View

Entropy measure and QSPR modeling in Graph Theor. How to construct the table for lengthy equation?

The entropy measured of molecular graphs plays a crucial rule. The network structures in some cases are very lengthy calculations to handle. Some author avoid to construct table where as most...

30 July 2024 3,126 0 View

"How has Leader Sheikh Hasina's government allegedly responded to student protests, including the reported killing of over 500 students ?

"How has Leader Sheikh Hasina's government allegedly responded to student protests, including the reported killing of over 500 students, as well as arrests, custody, remand, and the involvement of...

29 July 2024 2,181 7 View

Can a photocatalytic degradation of methylene blue from red mud be pseudo- zero order kinetics?

My photocatalyst from solid waste red med. Dye is methylene blue My all parameter study is showing zero order. How to prove it further that the reaction in zero order?

29 July 2024 7,404 1 View

Critique Calculaltion in Topological Indices and QSPR Modeling?

The first step is to analyze a 2D molecular graph and implement partitioning techniques to calculate the topological indices. Secondly impose statically tools to generate QSPR model, may or may...

24 July 2024 9,644 1 View

What strategy should be adopted to calculate topological indices efficiently?

Most of the time it is observed that in many manuscripts there are several typo error of topological calculation errors of calculation and partition of edges what ever the type of partitioning it...

22 July 2024 811 0 View

How to calculate pseudo order kinetics?

I know the rate law. But for my photo-catalytic experiments, first 90min is adsorption (dark) and next 150min is under UV light (total 240min). How to remove the adsorption part for calculation?...

21 July 2024 8,807 6 View

How to avoid errors while doing qpcr?

I have been doing qpcr for an year now but everytime I am getting errors. Either positive control cq values will vary or there will be no amolification. The knockdown genes are showing increased...

07 June 2024 7,292 1 View

Lentiviral Transduction Issues?

Hi there, I am attempting to transduce cells with the TLCV2 plasmid containing our sgRNA of interest. My transfection of the HEK 293T cells seems to yield positive results as the EGFP is clearly...

06 June 2024 2,131 1 View

My transduced cell can resist Puromycin and 99% express GFP, BUT can not show me the difference in the knockdown. what could be the possible reason?

My transduced cell can resist Puromycin and 99% express GFP, but it cannot show me the difference in the knockdown. What could be the possible reason?

31 May 2024 454 2 View

I will like to knockdown two genes using shRNA what is the best method and plasmid I can use?

21 May 2024 5,796 0 View

SiRNA services in India?

I am starting out on some siRNA based knockdowns on primary cells. Would anyone recommend good services they have used in India and any advice considering the advantage of modified and fluorophore...

09 May 2024 6,433 0 View

Effects of S100A14 knockdown on cancer cell proliferation and viability?

Above is a project am working on but feel kind of stuck.

21 March 2024 8,579 0 View

When you guys want to deplete bmp4, you usually do not use bmp4 gene knockdown directly?

I recently start and try to research the Bone morphogenetic protein 4 (bmp4) gene. I want to inhibit BMP4 gene expression and so I think bmp4 gene knockdown using si or shRNA is okay. but when I...

08 March 2024 5,196 0 View

How to research the function of a gene in cell?

Hello! I'm new in cell biology research. i have question about methodology to study cell growth. When comparing control cells with gene knockout cells, I found that the gene knockdown...

01 March 2024 3,467 3 View

CRISPR knockout cells showing expression again?

Hi all. I performed CRISPR knockout by lentiviral transduction. Cells were grown in puromycin for a period of time, protein was extracted and cells were also frozen down. A western blot showed...

24 February 2024 1,550 1 View

How to find out if a gene regulate's the transcription of other genes?

Hello all, I have transfected my cells with plasmid containing my shRNA sequences of my gene of interest and I'm looking this effect on 3 other genes, that when my gene is knockdown what happens...

08 January 2024 3,016 6 View