What kind of tubes can I use to do pellet culture?

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How to transfect circular mRNA to T cells?

Hello! We are using LNP to deliver circular mRNA into T cells. However, compared with linear mRNA, the transfection efficiency and protein expression duration are similar. Are LNP generation or...

21 July 2024 4,322 1 View

How to eliminate imaginary phonon frequency in phonon dispersion of Coved Graphene Nanoribbons?

Dear all, I want to calculate the phonon dispersion of 4CNR-1-0 (structure is in Fig. 2 (b) in this article Designing coved graphene nanoribbons with charge carrier mob... ) and then calculate...

17 June 2024 6,911 2 View

Mutagenesis question, 9 mismatch pcr cycle?

Dear all, I am currently trying to perform mutagenesis to change 9 mismatch. Is it possible to do it in one round of PCR? if so, are there any protocol I can learn from?

16 June 2024 875 2 View

Is it correct to call model 2 (Hayes Process) a double moderation model?

I am using PROCESS (model 2) to conduct moderation analysis with two moderators (moderator W is a categorical variable while moderator Z is a continuous variable). DV is predicted by X (IV), W, Z,...

07 May 2024 8,042 0 View

Failure to deliver mRNA to T cells via LNP, how to solve this problem?

When we used LNP to deliver GFP mRNA to Jurkat cells, the efficiency was as high as 90%. However, when we delivered GFP mRNA to T cells, we failed, with an efficiency of 0.5-0.6%. Could anyone...

06 March 2024 3,341 2 View

Zebra Finch Craniotomy?

Where is the HVC beside the letter Y in Zebra Finch's brain craniotomy?

25 February 2024 2,623 0 View

How to autoclave micro centrifuge tube?

Hi, I need to autoclave my sample (only 400ul) in micro centrifuge tube. Does anyone know how to autoclave it? Thanks!

22 February 2024 9,216 0 View

What are the similarities and differences between radiation and irradiation?

There is some confusion in the use of these two words.

05 February 2024 7,110 5 View

How to investigate the mechinism of transcrition factor and genes?

Hi, I have fond a strong negative correlation between a transcription factor and genes. How could I further decipher the underling mechanism? Thank you

23 January 2024 7,056 8 View

How to separate dead C.elegans from living ones?

The dead and alive C.elegans is mixed on one NGM plate, how can I separate alive one from dead one in a very rapid way? Besides , those living C.elegans has very low athletic ability.

10 January 2024 4,484 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View