How to separate dead C.elegans from living ones?

11 January 2024 1 4K Report

The dead and alive C.elegans is mixed on one NGM plate, how can I separate alive one from dead one in a very rapid way?

Besides , those living C.elegans has very low athletic ability.

To quickly distinguish between live and dead C. elegans on a mixed NGM plate, you can use a technique called "gentle touch." Live worms will respond to touch by moving, while dead ones will remain motionless. Gently touch the worms with a platinum wire or an eyelash pick, and observe their response to identify the live individuals.

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