Hi,
I am completely new to experimental techniques. What kind of questions can be answered if we have the images of different staining for example: GFP, EDU, DAPI, LAMIN etc.?
Can we measure the amount of chromatin from EDU or DAPI stained nuclei? or boundary of membrane from GFP?
Please suggest your view on theses areas or specific paper which would be relevant here.
Thanks.
Simple, but effective DAPI-based method for chromatin structure analysis:
Article Common Pathway of Chromosome Condensation in Mammalian Cells
Hi Ankit,
You need to be very specific about your questions in microscopy (best for qualitative answers) and of course the questions that you are asking can be answered.
What you want to study exactly? I may help you
Let me know, will be happy to share my experiences
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