Hello everyone,
Does anyone knows where people have calculated the volume of human chromosomes in a nuclei??
I have a tif image of size around ~10 Gb. I need to perform object classification or pixel classification in this image. The dimension of image data has zyx form. My voxel size in x=0.6, y=0.6 and...
31 December 2018 9,066 8 View
Dear all, I am using LAMMPS for coarse grain simulation of polymer chains. I want to control the temperature of the chains using thermostat. Which one should I chose? fit nvt or fix langevin...
08 September 2018 711 3 View
Hi, What is live Fluorescent In-situ hybridization imaging method? and how it is done?
01 February 2018 7,183 1 View
Hi, Nowadays, there are so many RNA detected so it is difficult to keep track of all the RNAs. Recently, I read about enhancer RNA that it is part of noncoding trancripts. I also read about...
31 December 2017 2,551 4 View
Hi, Is there any way to deform the shape of spherical region in LAMMPS. If i am right then fix deform command only used for to deform the simulation box. Is anyone know how to deform the...
02 March 2017 3,330 0 View
Is any one know how to draw voronoi diagram in 3d. And how to compute volume and surface area of each cell. If any function is available in MATLAB for computing then its is good otherwise any...
09 October 2016 8,753 1 View
RNA-seq data in ENCODE are available in these localization: cell, cytosol, nucleus etc. Is that means (cell localization = cytosol + nucleus) The main rna extraction methods are long poly A+, long...
06 July 2015 8,404 3 View
I found a nice review about current big data of biology and tools. So, I would recommend to read this paper by everyone "Computational solutions for omics data" who are working in Computational...
05 June 2015 5,165 0 View
Can anyone explain me the difference between exons and transcripts and is this true that coding sequence (CDS) are the exons. Another question about the splicing suppose if you have E1, E2 and E3...
05 June 2015 7,632 5 View
Hi, I am completely new to experimental techniques. What kind of questions can be answered if we have the images of different staining for example: GFP, EDU, DAPI, LAMIN etc.? Can we measure the...
01 January 1970 6,216 2 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I'm currently working on a project where I need to plot the atom-projected band structure using GPAW. I've been able to calculate the band structure for my material, but I'm having trouble...
07 August 2024 269 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View