Analysis of 3d image data as 2d?

I have a tif image of size around ~10 Gb. I need to perform object classification or pixel classification in this image. The dimension of image data has zyx form. My voxel size in x=0.6, y=0.6 and...

31 December 2018 8,968 8 View

What is the difference between Langevin and Nose hoover thermostat?

Dear all, I am using LAMMPS for coarse grain simulation of polymer chains. I want to control the temperature of the chains using thermostat. Which one should I chose? fit nvt or fix langevin...

08 September 2018 551 3 View

What is enhancer RNAs?

Hi, Nowadays, there are so many RNA detected so it is difficult to keep track of all the RNAs. Recently, I read about enhancer RNA that it is part of noncoding trancripts. I also read about...

31 December 2017 2,466 4 View

Volume of Chromosomes ?

04 May 2017 8,215 0 View

How to deform sphere region in LAMMPS?

Hi, Is there any way to deform the shape of spherical region in LAMMPS. If i am right then fix deform command only used for to deform the simulation box. Is anyone know how to deform the...

02 March 2017 3,247 0 View

Voronoi tesselation in 3d ?

09 October 2016 8,676 1 View

Difference between long poly A+ RNA and total RNA ?

RNA-seq data in ENCODE are available in these localization: cell, cytosol, nucleus etc. Is that means (cell localization = cytosol + nucleus) The main rna extraction methods are long poly A+, long...

06 July 2015 8,287 3 View

Next Gen Sequencing, Software, bioinformatics tools?

I found a nice review about current big data of biology and tools. So, I would recommend to read this paper by everyone "Computational solutions for omics data" who are working in Computational...

05 June 2015 5,074 0 View

Question related to transcripts, exons and splicing?

Can anyone explain me the difference between exons and transcripts and is this true that coding sequence (CDS) are the exons. Another question about the splicing suppose if you have E1, E2 and E3...

05 June 2015 7,557 5 View

What kind of questions can be asked from the tissue images of various staining?

Hi, I am completely new to experimental techniques. What kind of questions can be answered if we have the images of different staining for example: GFP, EDU, DAPI, LAMIN etc.? Can we measure the...

01 January 1970 6,144 2 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

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22 February 2021 9,932 11 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

Is there a method for evaluating nanoparticle adhesion to epithelial tissue in vitro?

I want to measure how well nanoparticles can transfect cells based on NP adhesion properties. In the literature I find people do it in animal studies, and epithelial adhesion helps in transfection...

21 February 2021 7,791 3 View