RNA-seq data in ENCODE are available in these localization: cell, cytosol, nucleus etc. Is that means
(cell localization = cytosol + nucleus)
The main rna extraction methods are long poly A+, long poly A- and total RNA. see in details here
I wanted to know that long poly A+ and total RNA are the same or not. If they are different then what is the difference between them.
http://www.epigenomebrowser.org/ENCODE/otherTerms.html
Hi Ankit,
I am not an expert on that, but as far as I know, total RNA is all RNA and Poli A+ RNA is all RNA less ribosomal RNAs (rRNA), transference RNA (tRNA) and other small ubiquitous RNAs. Ninety five percent of total RNA consists of that RNA and only
If you are interested in RNA editing or miRNA, poly A+ RNA seq will lose many information,since only mRNA and ncRNA with poly A tail would be enriched.
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