Hi,
Nowadays, there are so many RNA detected so it is difficult to keep track of all the RNAs. Recently, I read about enhancer RNA that it is part of noncoding trancripts. I also read about promoter and enhancer regions in the DNA sequence. Does enhancer RNAs are the product of enhancer regions of DNA sequence? If it is yes then promoter RNAs should also be present, right?
Also, what is the major difference between GRO-seq and RNA-seq method? Both used for detecting transcripts levels right?
Hello,
Yes, enhancer RNAs are transcribed from the DNA sequence of an enhancer region.
GRO-seq derives from RNA-seq and it measures the rates of transcript and not the steady state RNA levels, by measuring the newly produced RNA. Homer here (http://homer.ucsd.edu/homer/ngs/groseq/groseq.html) has a tutorial if you want to try it. This publication Chapter Computational Approaches for Mining GRO-Seq Data to Identify...
is also interesting.
hi
Enhancer RNAs (eRNAs) represent a class of relatively short non-coding RNA molecules (50-2000 nucleotides) transcribed from the DNA sequence of enhancerregions.
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology
- Biomolecules
- Nucleic Acids
- RNA