Yashvi Sharma

3 Questions 2 Answers 0 Followers

Questions related from Yashvi Sharma

How do I get good transfection efficiency of siRNA in Mesenchymal stem cells using lipofectamine RNAiMAX?

I am trying to transfect primary cells- mesenchymal stem cells using lipofectamine RNAiMAX. For its standardization I'm using a scrambled siRNA which is FITC tagged in order to come down to a set...

02 August 2021 8,938 0 View

If I have the Ct values (qpcr) of a housekeeping gene and 2 other genes of interest, how to normalize this data?

I have Ct values of my housekeeping gene, and i have ct values of 2 genes of interest. I want to show the level of these genes relative to housekeeping gene and relative to each other. I basically...

26 May 2021 9,467 4 View

What kind of cell culture contaminant is this, rod like forming aggregates?

These are initially solitary rod like small structures floating in the media, and then they start forming aggregates. Could this be bacillus? How to deal with it.

30 March 2021 7,667 6 View