How can droplet particle type be enabled in ANSYS Fluent?

Hello, I'm trying to enable droplet particle type in Fluent. Energy eqn.s are turned on. However only inert and massless types are available. So, if there is anyone who faced this kind of problem...

22 May 2020 6,387 1 View

Does expired PaxGene tubes have effect on the cfDNA yield or quality?

I have been using a set of PaxGene tubes to collect blood samples from which I have been isolating cfDNA for NGS analysis. I need to collect more blood samples, but I noticed that the expiration...

09 March 2020 3,967 1 View

Which concentration measurement is reliable for cfDNA? Nanodrop or Qubit?

I am trying to isolate cfDNA from plasma samples (2-3 ml) for NGS. I get around 15 ng/ul concentrations of cfDNA from nanodrop but 10 times lower conc (1.4 ng/ul) from qubit dsDNA HS assay. Why...

22 July 2018 7,155 3 View

TRAP staining protocol TROUBLEshooting?

Hi everyone, I am trying to deal with an unreasonable problem with TRAP staining of differentiated RAW264.7 cells (RANKL induced). I was using a protocol for TRAP staining, and it worked perfectly...

12 March 2017 8,140 3 View

PFGE - DNAse contamination?

26 October 2016 9,269 3 View

Converging problem in transient heat analysis due to amplitude. Anybody has idea?

15 January 2016 3,765 2 View

Is there any publicly available benchmark dataset to check software code plagiarism?

A plagiarized code may be either an exact copy of the original code, or a variant, applying various textual transformations such as; - Changing the order of operands/operators in expressions,-...

02 February 2015 9,851 7 View

Can anyone tell me the ingredients of liver cancer stem cell media?

26 October 2014 9,662 3 View

Is there any study which includes the software component management in component-based embedded software?

08 October 2014 4,134 1 View

Can someone suggest another software instead of Turbolab 4.0?

07 September 2014 1,882 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View