Can we pellet down total cell protein extract with centrifugation without using ammonium sulphate?

cells are lysed in RIPA buffer and I want to use that extract for IP, As extract has NP-40, SDS, high salts it will interfere with the binding of Ab with desired protein. Dialysis is one method...

07 August 2014 5,409 5 View

How to establish short term primary culture from AML patient blood?

Please share the protocols which are tried for the same and explain the critical points to take care.e.g. How to get rid of other cell population apart from blast cells? What should be the culture...

03 April 2014 8,677 2 View

What is the theoretical blood plasma concentration of Mitoxantrone?

Mitoxantrone is given to patients as 12mg/m2 body surface area. If Molecular weight of Mitoxantrone is 517 then what could be the molar concentration of mitoxantrone in blood plasma in a patient...

01 February 2014 4,279 1 View

What is the medium for culture and maintenance of CD34+ and CD38- cells?

I want to culture stem cells but I don't know which is the best medium for it? And how long can I maintain them as stem cells?

31 December 2013 9,692 1 View

Is anybody interested in sharing chemo-resistant AML cell lines?

I am working on DNA damage and repair in chemoresistant AML. Developing resistant cell line is taking quite some time, so if any one wishes to share AML cell lines( eg. HL-60, THP-1, Kasumi-1 etc)...

31 December 2013 8,437 2 View

Why is there no therapeutic monoclonal antibody/ chemical inhibitor against different translocation proteins found in AML?

Just like we have imatinib against BCR-ABLfusion protein. Why dont we have any inhibitor or antibody against MLL, PML-RARA and AML- ETO?

31 December 2013 2,793 1 View

What could be the reason that primary heart tumors are very rare?

Even secondary tumors of heart are rare though metastatic cancers spreads through blood flow and blood is circulated by heart.

31 December 2013 6,429 1 View

Experience with cell cycle analysis using PI staining after doxorubicin treatment?

Doxorubicin is overlapping in FL-2 channel and the profile looks indistinct. Has anyone faced such problem? any solution?

11 December 2013 7,108 3 View

How can one make a drug resistant (DNA damaging drug) cell line?

I have been trying to make a drug resistant (Doxorubicin) cell line from HL-60 cells (Suspension culture) but some how I am not able to regrow the drug treated cells even after IC-50 treatment....

11 December 2013 5,943 3 View

Kasumi-1 cell line is not growing. Any suggestions?

I am trying to grow Kasumi-1 cell line. I use RPMI 1640 and 20 % FBS but cells never seem to be growing. They keep on shrinking in size and eventually die off.

11 December 2013 7,917 4 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View