What is the third most important utilizable source of carbon in cell culture after glucose and L-glutamine?

08 September 2014 8,635 5 View

Can we pellet down total cell protein extract with centrifugation without using ammonium sulphate?

cells are lysed in RIPA buffer and I want to use that extract for IP, As extract has NP-40, SDS, high salts it will interfere with the binding of Ab with desired protein. Dialysis is one method...

07 August 2014 5,409 5 View

How to establish short term primary culture from AML patient blood?

Please share the protocols which are tried for the same and explain the critical points to take care.e.g. How to get rid of other cell population apart from blast cells? What should be the culture...

03 April 2014 8,677 2 View

What is the theoretical blood plasma concentration of Mitoxantrone?

Mitoxantrone is given to patients as 12mg/m2 body surface area. If Molecular weight of Mitoxantrone is 517 then what could be the molar concentration of mitoxantrone in blood plasma in a patient...

01 February 2014 4,279 1 View

What is the medium for culture and maintenance of CD34+ and CD38- cells?

I want to culture stem cells but I don't know which is the best medium for it? And how long can I maintain them as stem cells?

31 December 2013 9,692 1 View

Is anybody interested in sharing chemo-resistant AML cell lines?

I am working on DNA damage and repair in chemoresistant AML. Developing resistant cell line is taking quite some time, so if any one wishes to share AML cell lines( eg. HL-60, THP-1, Kasumi-1 etc)...

31 December 2013 8,437 2 View

Why is there no therapeutic monoclonal antibody/ chemical inhibitor against different translocation proteins found in AML?

Just like we have imatinib against BCR-ABLfusion protein. Why dont we have any inhibitor or antibody against MLL, PML-RARA and AML- ETO?

31 December 2013 2,793 1 View

What could be the reason that primary heart tumors are very rare?

Even secondary tumors of heart are rare though metastatic cancers spreads through blood flow and blood is circulated by heart.

31 December 2013 6,429 1 View

Experience with cell cycle analysis using PI staining after doxorubicin treatment?

Doxorubicin is overlapping in FL-2 channel and the profile looks indistinct. Has anyone faced such problem? any solution?

11 December 2013 7,108 3 View

How can one make a drug resistant (DNA damaging drug) cell line?

I have been trying to make a drug resistant (Doxorubicin) cell line from HL-60 cells (Suspension culture) but some how I am not able to regrow the drug treated cells even after IC-50 treatment....

11 December 2013 5,943 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View