What is the third most important utilizable source of carbon in cell culture after glucose and L-glutamine?

08 September 2014 8,635 5 View

Can we pellet down total cell protein extract with centrifugation without using ammonium sulphate?

cells are lysed in RIPA buffer and I want to use that extract for IP, As extract has NP-40, SDS, high salts it will interfere with the binding of Ab with desired protein. Dialysis is one method...

07 August 2014 5,409 5 View

How to establish short term primary culture from AML patient blood?

Please share the protocols which are tried for the same and explain the critical points to take care.e.g. How to get rid of other cell population apart from blast cells? What should be the culture...

03 April 2014 8,677 2 View

What is the theoretical blood plasma concentration of Mitoxantrone?

Mitoxantrone is given to patients as 12mg/m2 body surface area. If Molecular weight of Mitoxantrone is 517 then what could be the molar concentration of mitoxantrone in blood plasma in a patient...

01 February 2014 4,279 1 View

What is the medium for culture and maintenance of CD34+ and CD38- cells?

I want to culture stem cells but I don't know which is the best medium for it? And how long can I maintain them as stem cells?

31 December 2013 9,692 1 View

Is anybody interested in sharing chemo-resistant AML cell lines?

I am working on DNA damage and repair in chemoresistant AML. Developing resistant cell line is taking quite some time, so if any one wishes to share AML cell lines( eg. HL-60, THP-1, Kasumi-1 etc)...

31 December 2013 8,437 2 View

Why is there no therapeutic monoclonal antibody/ chemical inhibitor against different translocation proteins found in AML?

Just like we have imatinib against BCR-ABLfusion protein. Why dont we have any inhibitor or antibody against MLL, PML-RARA and AML- ETO?

31 December 2013 2,793 1 View

Experience with cell cycle analysis using PI staining after doxorubicin treatment?

Doxorubicin is overlapping in FL-2 channel and the profile looks indistinct. Has anyone faced such problem? any solution?

11 December 2013 7,108 3 View

How can one make a drug resistant (DNA damaging drug) cell line?

I have been trying to make a drug resistant (Doxorubicin) cell line from HL-60 cells (Suspension culture) but some how I am not able to regrow the drug treated cells even after IC-50 treatment....

11 December 2013 5,943 3 View

Kasumi-1 cell line is not growing. Any suggestions?

I am trying to grow Kasumi-1 cell line. I use RPMI 1640 and 20 % FBS but cells never seem to be growing. They keep on shrinking in size and eventually die off.

11 December 2013 7,917 4 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

No title

02 March 2021 3,060 3 View

No title

02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View