Sameer Jagannathrao Salunkhe

12 Questions 9 Answers 0 Followers

Questions related from Sameer Jagannathrao Salunkhe

What is the third most important utilizable source of carbon in cell culture after glucose and L-glutamine?

I am using RPMI 1640 and found that glucose and L-glutamine are not consumed. Many cells are still happily growing. Can there be a change in metabolic preferences?

09 September 2014 8,705 5 View

Can we pellet down total cell protein extract with centrifugation without using ammonium sulphate?

cells are lysed in RIPA buffer and I want to use that extract for IP, As extract has NP-40, SDS, high salts it will interfere with the binding of Ab with desired protein. Dialysis is one method...

08 August 2014 5,497 5 View

How to establish short term primary culture from AML patient blood?

Please share the protocols which are tried for the same and explain the critical points to take care.e.g. How to get rid of other cell population apart from blast cells? What should be the culture...

04 April 2014 8,749 2 View

What is the theoretical blood plasma concentration of Mitoxantrone?

Mitoxantrone is given to patients as 12mg/m2 body surface area. If Molecular weight of Mitoxantrone is 517 then what could be the molar concentration of mitoxantrone in blood plasma in a patient...

02 February 2014 4,354 1 View

Is anybody interested in sharing chemo-resistant AML cell lines?

I am working on DNA damage and repair in chemoresistant AML. Developing resistant cell line is taking quite some time, so if any one wishes to share AML cell lines( eg. HL-60, THP-1, Kasumi-1 etc)...

01 January 2014 8,494 2 View

What is the medium for culture and maintenance of CD34+ and CD38- cells?

I want to culture stem cells but I don't know which is the best medium for it? And how long can I maintain them as stem cells?

01 January 2014 9,744 1 View

Why is there no therapeutic monoclonal antibody/ chemical inhibitor against different translocation proteins found in AML?

Just like we have imatinib against BCR-ABLfusion protein. Why dont we have any inhibitor or antibody against MLL, PML-RARA and AML- ETO?

01 January 2014 2,858 1 View

What could be the reason that primary heart tumors are very rare?

Even secondary tumors of heart are rare though metastatic cancers spreads through blood flow and blood is circulated by heart.

01 January 2014 6,487 1 View

Experience with cell cycle analysis using PI staining after doxorubicin treatment?

Doxorubicin is overlapping in FL-2 channel and the profile looks indistinct. Has anyone faced such problem? any solution?

12 December 2013 7,172 3 View

Kasumi-1 cell line is not growing. Any suggestions?

I am trying to grow Kasumi-1 cell line. I use RPMI 1640 and 20 % FBS but cells never seem to be growing. They keep on shrinking in size and eventually die off.

12 December 2013 7,996 4 View

How can one make a drug resistant (DNA damaging drug) cell line?

I have been trying to make a drug resistant (Doxorubicin) cell line from HL-60 cells (Suspension culture) but some how I am not able to regrow the drug treated cells even after IC-50 treatment....

12 December 2013 6,005 3 View

Does lipofectamine 2000 give a stable transfection?

Do Lipofectamine 2000 transfected plasmids get integrated into chromosomes? And if not then how many generations does it remain inside the transfected cell? If transient then how transient?

12 December 2013 8,432 5 View