What is the suitable concentration of templete for qPCR?

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In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

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How to get onecolumn document from standard IEEE ACCESS LateX template?

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Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

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How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

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01 March 2021 2,027 2 View

OpenBCI eeg--has anyone used them?

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How to remove DCU and HOBT from my reaction?

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28 February 2021 2,153 1 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

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How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

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POlymer+DMSO solution and moisture??

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B-mercaptoethanol (BME) protocol?

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27 February 2021 5,028 2 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

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03 March 2021 687 1 View

How do I increase the yield of my PCR product?

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02 March 2021 4,029 3 View

How to quantitative analize the data of TNBS assay for gelatin?

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Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

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01 March 2021 5,035 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

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How to address this issue in the analysis of Real-Time PCR results?

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No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Distribution of particles in metal matrix?

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MiRNA qpcr problem?

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28 February 2021 5,008 4 View

Noor Idan Jarad

hi see thi link

https://www.researchgate.net/.../How_much_DNA_template_genomic_or_plasmid_D

Asst.Prof.Muthanna Hadi Hussain

I think it due to the treatment...

Ilkka Nousiainen

Hi,

I have used 10ng/µl template concentration and succesfully detected differences in amplification between samples, so I don't think that increasing template concentration helps in this case. The simplest conclusion is that there is no difference between treatment group and control group.