16 Questions 26 Answers 0 Followers
Questions related from Sadia Kanwal
Can we calculate p value of PCoA using SPSS or graphpad prism? Which test is recommended for PCoA analysis? Which software is required to perform PERMANOVA or ANOSIM? is it possible to perform...
16 June 2020 7,155 3 View
I have a question regarding sequencing analysis. I sent my PCR product for conventional Sanger sequencing.Initially I did DGGE for microbial profiling and cut some of the specific bands for...
26 February 2018 7,170 4 View
What are the basis for the selection of internal control in western blotting? I am quite clear about the controls for nuclear, mitochondrial and cytosolic fractions, what i would like to know is,...
16 February 2018 5,548 5 View
Is there any free software available online for PCoA analysis? please suggest. Regards
14 February 2018 6,729 7 View
What are the critical physico-chemical characterization that should be considered for a novel polysaccharide isolated from mushroom? Thanks
12 November 2017 8,914 3 View
How to determine if a particular drug, plant, mushroom or a component of mushroom (polysaccharides, protein etc) is a patent? I tried doing by Google, but still confuse whether its the right way...
15 October 2017 1,928 4 View
what is the purpose of doing methylation analysis for the polysaccharide fractions that are extracted from mushroom using acid or alkali method. Thanks .
10 October 2017 3,406 3 View
I want to purify crude polysaccharide using any efficient chromatographic method. which resin is ideal for Polysaccharide purification ? Please suggest. Thanks in advance. Regards.
28 September 2017 467 9 View
Two bands of Bcl-2 are appearing on my western blot, what could be the possible reason for that? is it normal?
14 July 2017 2,431 6 View
Hello while doing mitochondrial membrance potential assay b JC-1, what could be the best positive control? Thanks
03 July 2017 8,823 4 View
After DSF guided refolding of protein, results for denatured and refolded samples are coming out like this. is it ok if the denatured sample give peak and Tm like this? as i have used mild...
24 June 2017 1,909 3 View
how to validate the transfer condition (time/V or mA) for proteins of different molecular weight in western blotting?what could be the best transfer condition for proteins 14-30 KDa and 30-100KDa?
07 June 2017 7,827 2 View
Which non-radioactive kit can be used for in vitro translation inhibition assay? I am searching this kit but unable to find any related literature ( non-radioactive rabbit reticulocyte lysate...
26 May 2017 677 2 View
I have used 20ng final concentration of template in my qPCR experiment, but not getting any difference between the Ct values of control and test group after drug treatment. Should i increase the...
26 May 2017 508 3 View
I have seen in literature, people have shown figures of transwell for migration and invasion assay (suspension cells) but as far as i know, when we will try to fix the suspension cells they will...
26 May 2017 4,969 3 View
I'm working on Dictyophora indusiata mushroom and í need to deproteinize crude polysaccharide.I have looked into literature but didn't got any precise protocol. If any one has exact protocol...
13 April 2017 1,655 18 View
