In order to grow cells in the presence of a miRNA What is the miRNAs structure should I use, could be the same structure of miRNAs that exists in serum and where can I order it.
It has been revealed that several miRNA-binding protein are involved in circulating miRNA inter-or extra-cellular communication, such as nucleophosmin1 (NPM1) and Argonaute2 (Ago2) -1,-2 -3 and -4. Further, the uptake of miRNA high-density lipoprotein (HDL) in plasma by recipient cells appears to be dependent on a cell-surface HDL receptor named scavenger receptor class B, type I (SRBI).
Circulating miRNAs have been shown to be carried within extracellular vesicles (exosomes, microvesicles, and apoptotic bodies) or bound to HDL particles and Argonaute protein. If you are trying to evaluate the effects of a specific miRNA in cultured cells, you could perhaps use miRNA mimics. Some options for commercially available miRNA mimics: http://www.exiqon.com/mirna-mimics http://www.lifetechnologies.com/uk/en/home/life-science/epigenetics-noncoding-rna-research/mirna-profiling-/mirvana-mimics-inhibitors.html. I hope this could help.
you can use mirVana mimics- they are synthetically produced and closely resemble natural miRNAs
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