I want to know if it is possible to make acridine orange staining (to detect apoptosis) on cells resulting from enzymatic digestion of tissue by dispase and preserved with acetic acid methanol.
Thanks
Yes, Acridine orange is an intercalating dye that can permeate both live and fixed cells.
Yes, it is possible to perform acridine orange staining on cells resulting from enzymatic digestion of tissue by dispase and preserved with acetic acid methanol. Acridine orange is a fluorescent dye commonly used to detect apoptosis in cells. Enzymatic digestion using dispase helps to dissociate the cells from the tissue matrix, allowing for the analysis of individual cells. Preserving the cells with acetic acid methanol helps to fix and preserve the cellular structures, enabling subsequent staining procedures. Acridine orange staining can then be applied to these cells to visualize apoptotic features, such as fragmented DNA or condensed chromatin, under a fluorescence microscope. This staining method can provide valuable information about the occurrence of apoptosis in the cells resulting from the enzymatic digestion of tissue.
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